Rabbit anti-Human KRT35 Polyclonal Antibody | anti-KRT35 antibody

KRT35, CT (KRT35, HHA5, HKA5, KRTHA5, Keratin, type I cuticular Ha5, Hair keratin, type I Ha5, Keratin-35) (MaxLight 405)

Cat. Num. AAA6314683

• Gene Names: KRT35; HA5; Ha-5; hHa5; KRTHA5
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: KRT35; Polyclonal Antibody; CT (KRT35; HHA5; HKA5; KRTHA5; Keratin; type I cuticular Ha5; Hair keratin; type I Ha5; Keratin-35) (MaxLight 405); Keratin-35; anti-KRT35 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KRT35.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-KRT35 antibody: Western Blot (WB), FLISA, Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 358-380 in the C-terminal region of human KRT35, UniProt Accession #Q92764.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ZR-75-1 cell line lysates (35ug/lane) usingMBS645308. KRT35 (arrow) was detected usingMBS645308.)

Immunohistochemistry (IHC)

(IHC analysis in formalin fixed and paraffin embedded skin carcinoma using MBS645308 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS645308 for immunohistochemistry.)

Related Product Information for anti-KRT35 antibody

KRT35 is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription.

Product Categories/Family for anti-KRT35 antibody

Antibodies; Keratin; Cytokeratin

References

1. Gilon, M., et al. Differentiation 76(5):518-530(2008). 2. Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006). 3. Cribier, B., et al. J. Invest. Dermatol. 122(5):1078-1083(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 94538345
• NCBI GeneID: 3886
• NCBI Accession #: NP_002271
• NCBI GenBank Nucleotide #: NM_002280.4
• UniProt Accession #: Q92764; O76012; Q92651
• Molecular Weight: 50,361 Da
• NCBI Official Full Name: keratin, type I cuticular Ha5
• NCBI Official Synonym Full Names: keratin 35
• NCBI Official Symbol: KRT35
• NCBI Official Synonym Symbols: HA5; Ha-5; hHa5; KRTHA5
• NCBI Protein Information: keratin, type I cuticular Ha5
• UniProt Protein Name: Keratin, type I cuticular Ha5
• Protein Family: Keratin
• UniProt Gene Name: KRT35
• UniProt Synonym Gene Names: HHA5; HKA5; KRTHA5; K35
• UniProt Entry Name: KRT35_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. [provided by RefSeq, Jul 2008]

Uniprot Description

KRTHA5: is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. [provided by RefSeq, Jul 2008]

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: extracellular space; intermediate filament

Molecular Function: structural molecule activity

Biological Process: anatomical structure morphogenesis