Rabbit anti-Human KRT25 Polyclonal Antibody | anti-KRT25 antibody

KRT25, CT (KRT25, KRT25A, Keratin, type I cytoskeletal 25, Cytokeratin-25, Keratin-25, Keratin-25A, Type I inner root sheath-specific keratin-K25irs1) (FITC)

Cat. Num. AAA6314615

• Gene Names: KRT25; KRT25A
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KRT25; Polyclonal Antibody; CT (KRT25; KRT25A; Keratin; type I cytoskeletal 25; Cytokeratin-25; Keratin-25; Keratin-25A; Type I inner root sheath-specific keratin-K25irs1) (FITC); Type I inner root sheath-specific keratin-K25irs1; anti-KRT25 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-KRT25 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KRT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-426 amino acids from the C-terminal region of human KRT25.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KRT25 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the KRT25 antibody detected the KRT25 protein (arrow).)

Flow Cytometry (FC/FACS)

(KRT25 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KRT25 antibody

This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21.

Product Categories/Family for anti-KRT25 antibody

Antibodies; Cytokeratin

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006). Rogers, M.A., et al. Differentiation 72 (9-10), 527-540 (2004): Hesse, M., et al. Eur. J. Cell Biol. 83(1):19-26(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 31559829
• NCBI GeneID: 147183
• NCBI Accession #: NP_853512.1
• NCBI GenBank Nucleotide #: NM_181534.3
• UniProt Accession #: Q7Z3Z0
• Molecular Weight: 49,318 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 25
• NCBI Official Synonym Full Names: keratin 25, type I
• NCBI Official Symbol: KRT25
• NCBI Official Synonym Symbols: KRT25A
• NCBI Protein Information: keratin, type I cytoskeletal 25
• UniProt Protein Name: Keratin, type I cytoskeletal 25
• Protein Family: Keratin
• UniProt Gene Name: KRT25
• UniProt Synonym Gene Names: CK-25; K25; K25A
• UniProt Entry Name: K1C25_HUMAN

NCBI Description

This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2009]

Uniprot Description

KRT25: Essential for the proper assembly of type I and type II keratin protein complexes and formation of keratin intermediate filaments in the inner root sheath (irs). Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; intermediate filament

Molecular Function: structural molecule activity

Biological Process: hair follicle morphogenesis; intermediate filament organization; aging; hair cycle