<
IHC (Immunohistchemistry)
(KRT18/CK18/Cytokeratin 18 Antibody-Immunohistochemical analysis of Cytokeratin 18 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.)
Rabbit anti-Human KRT18/CK18/Cytokeratin 18 Polyclonal Antibody | anti-KRT18 antibody
KRT18/CK18/Cytokeratin 18 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Gene Names
KRT18; K18; CYK18
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Immunoaffinity purified
Synonyms
KRT18/CK18/Cytokeratin 18; Polyclonal Antibody; KRT18/CK18/Cytokeratin 18 Rabbit anti-Human Polyclonal (C-Terminus) Antibody; IHC-plus KRT18/CK18/Cytokeratin 18 Antibody (C-Terminus); KRT18; CK-18; Cytokeratin 18; Cytokeratin-18; K18; Keratin 18; CYK18; Keratin-18; anti-KRT18 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Cytokeratin 18 protein.
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, pH7.3, 0.01% Sodium Azide, 30% Glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-KRT18 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Target
Human KRT18/CK18/Cytokeratin 18
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human Cytokeratin 18.
Conjugation
Unconjugated
Epitope
C-Terminus
Family
Keratin
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
IHC (Immunohistchemistry)
(KRT18/CK18/Cytokeratin 18 Antibody-Immunohistochemical analysis of Cytokeratin 18 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.)
IHC (Immunohistchemistry)
(KRT18/CK18/Cytokeratin 18 Antibody-Immunohistochemical analysis of Cytokeratin 18 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.)
IHC (Immunohistochemistry)
(KRT18/CK18/Cytokeratin 18 Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(KRT18/CK18/Cytokeratin 18 Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(KRT18/CK18/Cytokeratin 18 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(KRT18/CK18/Cytokeratin 18 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(KRT18/CK18/Cytokeratin 18 Antibody-Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(KRT18/CK18/Cytokeratin 18 Antibody-Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(KRT18/CK18/Cytokeratin 18 Antibody-Western blot analysis of Cytokeratin 18 expression in HeLa (A); A431 (B); MCF7 (C) whole cell lysates.)
WB (Western Blot)
(KRT18/CK18/Cytokeratin 18 Antibody-Western blot analysis of Cytokeratin 18 expression in HeLa (A); A431 (B); MCF7 (C) whole cell lysates.)
ICC (Immunocytochemistry)
(KRT18/CK18/Cytokeratin 18 Antibody-Immunofluorescent analysis of Cytokeratin 18 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
ICC (Immunocytochemistry)
(KRT18/CK18/Cytokeratin 18 Antibody-Immunofluorescent analysis of Cytokeratin 18 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Related Product Information for anti-KRT18 antibody
Cytokeratin 18 antibody is an unconjugated rabbit polyclonal antibody to human Cytokeratin 18 (KRT18/CK18)(C-Terminus). Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,058 Da
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
KRT18
NCBI Official Synonym Symbols
K18; CYK18
NCBI Protein Information
keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein
UniProt Protein Name
Keratin, type I cytoskeletal 18
UniProt Gene Name
KRT18
UniProt Synonym Gene Names
CYK18; CK-18; K18
UniProt Entry Name
K1C18_HUMAN
Similar Products
Product Notes
The KRT18 krt18 (Catalog #AAA21349) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KRT18/CK18/Cytokeratin 18 Rabbit anti-Human Polyclonal (C-Terminus) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KRT18/CK18/Cytokeratin 18 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB). Researchers should empirically determine the suitability of the KRT18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT18/CK18/Cytokeratin 18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
