Rabbit anti-Human KRT15 Polyclonal Antibody | anti-KRT15 antibody

KRT15 (Keratin, Type I Cytoskeletal 15, Cytokeratin-15, CK-15, Keratin-15, K15, KRTB) (FITC)

Cat. Num. AAA6383842

• Gene Names: KRT15; K15; CK15; K1CO
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KRT15; Polyclonal Antibody; KRT15 (Keratin; Type I Cytoskeletal 15; Cytokeratin-15; CK-15; Keratin-15; K15; KRTB) (FITC); anti-KRT15 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KRT15.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-KRT15 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human KRT15, aa1-456 (NP_002266.2).
• Immunogen Sequence: MTTTFLQTSSSTFGGGSTRGGSLLAGGGGFGGGSLSGGGGSRSISASSARFVSSGSGGGYGGGMRVCGFGGGAGSVFGGGFGGGVGGGFGGGFGGGDGGLLSGNEKITMQNLNDRLASYLDKVRALEEANADLEVKIHDWYQKQTPTSPECDYSQYFKTIEELRDKIMATTIDNSRVILEIDNARLAADDFRLKYENELALRQGVEADINGLRRVLDELTLARTDLEMQIEGLNEELAYLKKNHEEEMKEFSSQLAGQVNVEMDAAPGVDLTRVLAEMREQYEAMAEKNRRDVEAWFFSKTEELNKEVASNTEMIQTSKTEITDLRRTMQELEIELQSQLSMKAGLENSLAETECRYATQLQQIQGLIGGLEAQLSELRCEMEAQNQEYKMLLDIKTRLEQEIATYRSLLEGQDAKMAGIGIREASSGGGGSSSNFHINVEESVDGQVVSSHKREI
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KRT15 rabbit polyclonal antibody. Western Blot analysis of KRT15 expression in A-431.)

Western Blot (WB)

(Western Blot analysis of KRT15 expression in transfected 293T cell line by KRT15 polyclonal antibody. Lane 1: KRT15 transfected lysate (49.2kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-KRT15 antibody

Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate.

Product Categories/Family for anti-KRT15 antibody

Antibodies; Keratin; Cytokeratin

NCBI and Uniprot Product Information

• NCBI GI #: 24430190
• NCBI GeneID: 3866
• NCBI Accession #: NP_002266.2
• NCBI GenBank Nucleotide #: NM_002275.2
• UniProt Accession #: P19012; Q53XV8; Q9BUG4; B3KQY1
• Molecular Weight: 49,212 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 15
• NCBI Official Synonym Full Names: keratin 15
• NCBI Official Symbol: KRT15
• NCBI Official Synonym Symbols: K15; CK15; K1CO
• NCBI Protein Information: keratin, type I cytoskeletal 15; CK-15; cytokeratin 15; cytokeratin-15; keratin-15, beta; keratin-15, basic
• UniProt Protein Name: Keratin, type I cytoskeletal 15
• Protein Family: Keratin
• UniProt Gene Name: KRT15
• UniProt Synonym Gene Names: KRTB; CK-15; K15
• UniProt Entry Name: K1C15_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq, Jul 2008]

Uniprot Description

K15: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: intermediate filament; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: epidermis development