Rabbit anti-Human KRT13 Polyclonal Antibody | anti-KRT13 antibody

KRT13, NT (Keratin, Type I Cytoskeletal 13, Cytokeratin-13, CK-13, Keratin-13, K13) (PE)

Cat. Num. AAA6484227

• Gene Names: KRT13; K13; CK13; WSN2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: KRT13; Polyclonal Antibody; NT (Keratin; Type I Cytoskeletal 13; Cytokeratin-13; CK-13; Keratin-13; K13) (PE); anti-KRT13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KRT13.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-KRT13 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human KRT13 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KRT13 antibody (N-term) in 293 cell line lysates (35ug/lane). KRT13 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(KRT13 Antibody (N-term) (RB18903) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KRT13 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(KRT13 Antibody (N-term) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-KRT13 antibody

Antibodies; Keratin; Cytokeratin

References

1. Sheng, S., Mol. Cell. Endocrinol. 296(1-2), 1-9 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 30377
• NCBI GeneID: 3860
• NCBI Accession #: NP_002265
• NCBI GenBank Nucleotide #: NM_002274
• UniProt Accession #: P13646
• Molecular Weight: 46kDa
• NCBI Official Full Name: cytokeratin 13
• NCBI Official Synonym Full Names: keratin 13
• NCBI Official Symbol: KRT13
• NCBI Official Synonym Symbols: K13; CK13; WSN2
• NCBI Protein Information: keratin, type I cytoskeletal 13
• UniProt Protein Name: Keratin, type I cytoskeletal 13
• Protein Family: Keratin
• UniProt Gene Name: KRT13
• UniProt Synonym Gene Names: CK-13; K13

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

MiscellaneousThere are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).