Western Blot (WB)
(Western blot analysis of KRT12 using Jurkat whole cell lysates)
Rabbit anti-Human, Mouse KRT12 Polyclonal Antibody | anti-KRT12 antibody
KRT12 Antibody
Gene Names
KRT12; K12
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
KRT12; Polyclonal Antibody; KRT12 Antibody; CK-12; Cytokeratin-12; K12; K1C12_HUMAN; Keratin 12; Keratin; type I cytoskeletal 12; Keratin-12; anti-KRT12 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
KRT12 antibody detects endogenous levels of total KRT12
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
494
Applicable Applications for anti-KRT12 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human KRT12
Tissue Specificity
Cornea specific.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog
Similarity
Pig (82%), Bovine (82%), Sheep (82%), Rabbit (91%), Dog (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of KRT12 using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis of KRT12 using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis of extracts from 293, using KRT12 Antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293, using KRT12 Antibody.)
Related Product Information for anti-KRT12 antibody
Function: May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity (By similarity).
Subunit Structure: Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.
Similarity: Belongs to the intermediate filament family.
Subunit Structure: Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.
Similarity: Belongs to the intermediate filament family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 54 kDa
Predicted: 54 kDa
Predicted: 54 kDa
NCBI Official Full Name
keratin, type I cytoskeletal 12
NCBI Official Synonym Full Names
keratin 12
NCBI Official Symbol
KRT12
NCBI Official Synonym Symbols
K12
NCBI Protein Information
keratin, type I cytoskeletal 12
UniProt Protein Name
Keratin, type I cytoskeletal 12
Protein Family
UniProt Gene Name
KRT12
UniProt Synonym Gene Names
CK-12; K12
NCBI Description
KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq, Jul 2008]
Uniprot Description
May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity ().
Research Articles on KRT12
Similar Products
Product Notes
The KRT12 krt12 (Catalog #AAA9608709) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KRT12 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KRT12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the KRT12 krt12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
