Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of KRT10 Antibody (N-term) in 293 cell line lysates (35ug/lane). KRT10 (arrow) was detected using the purified Pab.)

Rabbit anti-Human KRT10 Polyclonal Antibody | anti-KRT10 antibody

KRT10, NT (Keratin, Type I Cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KPP) (AP)

Gene Names
KRT10; BIE; EHK; K10; KPP; BCIE; CK10
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
KRT10; Polyclonal Antibody; NT (Keratin; Type I Cytoskeletal 10; Cytokeratin-10; CK-10; Keratin-10; K10; KPP) (AP); anti-KRT10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human KRT10.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-KRT10 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human KRT10 (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KRT10 Antibody (N-term) in 293 cell line lysates (35ug/lane). KRT10 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of KRT10 Antibody (N-term) in 293 cell line lysates (35ug/lane). KRT10 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human kidney carcinoma with KRT10 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human kidney carcinoma with KRT10 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells using KRT10 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of 293 cells using KRT10 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-KRT10 antibody
References
1. Morais, P., Eur J Dermatol 19(4), 333-336 (2009). 2. Barcelos, A.C., J. Cutan. Pathol. 36(6), 647-654 (2009).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
58,827 Da
NCBI Official Full Name
keratin, type I cytoskeletal 10
NCBI Official Synonym Full Names
keratin 10
NCBI Official Symbol
KRT10
NCBI Official Synonym Symbols
BIE; EHK; K10; KPP; BCIE; CK10
NCBI Protein Information
keratin, type I cytoskeletal 10; CK-10; keratin-10; cytokeratin 10
UniProt Protein Name
Keratin, type I cytoskeletal 10
Protein Family
UniProt Gene Name
KRT10
UniProt Synonym Gene Names
KPP; CK-10; K10
UniProt Entry Name
K1C10_HUMAN

Uniprot Description

K10: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K1.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extracellular space; membrane; keratin filament; cytoplasm; intermediate filament; nucleus

Molecular Function: structural constituent of epidermis

Biological Process: keratinocyte differentiation

Disease: Erythroderma, Ichthyosiform, Congenital Reticular; Epidermolytic Hyperkeratosis; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis

Similar Products

Product Notes

The KRT10 krt10 (Catalog #AAA6484195) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KRT10, NT (Keratin, Type I Cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KPP) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KRT10 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KRT10 krt10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.