Rabbit anti-Human KPNA5 Polyclonal Antibody | anti-KPNA5 antibody

KPNA5, NT (KPNA5, Importin subunit alpha-6, Karyopherin subunit alpha-5) (APC)

Cat. Num. AAA6314415

• Gene Names: KPNA5; SRP6; IPOA6
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KPNA5; Polyclonal Antibody; NT (KPNA5; Importin subunit alpha-6; Karyopherin subunit alpha-5) (APC); Karyopherin subunit alpha-5; anti-KPNA5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-KPNA5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KPNA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human KPNA5.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(KPNA5 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the KPNA5 antibody detected the KPNA5 protein (arrow).)

Related Product Information for anti-KPNA5 antibody

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus.

Product Categories/Family for anti-KPNA5 antibody

Antibodies; Proteins

References

Yang, S.N., et al. J. Biol. Chem. 285(26):19935-19946(2010). Singh, A.P., et al. Cell 131(3):492-504(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Lim, J., et al. Cell 125(4):801-814(2006). Sekimoto, T., et al. EMBO J. 23(9):1934-1942(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 39812229
• NCBI GeneID: 3841
• NCBI Accession #: NP_002260.2
• NCBI GenBank Nucleotide #: NM_002269.2
• UniProt Accession #: O15131; Q86X23; B2RAI5
• Molecular Weight: 60,349 Da
• NCBI Official Full Name: importin subunit alpha-6
• NCBI Official Synonym Full Names: karyopherin alpha 5 (importin alpha 6)
• NCBI Official Symbol: KPNA5
• NCBI Official Synonym Symbols: SRP6; IPOA6
• NCBI Protein Information: importin subunit alpha-6; importin alpha 6; karyopherin subunit alpha-5
• UniProt Protein Name: Importin subunit alpha-6
• UniProt Gene Name: KPNA5
• UniProt Entry Name: IMA6_HUMAN

NCBI Description

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus. [provided by RefSeq, Jul 2008]

Uniprot Description

KPNA5: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates nuclear import of STAT1 homodimers and STAT1/STAT2 heterodimers by recognizing non- classical NLSs of STAT1 and STAT2 through ARM repeats 8-9. Recognizes influenza A virus nucleoprotein through ARM repeat 7-9 In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Belongs to the importin alpha family.

Protein type: Nuclear import; Karyopherin

Chromosomal Location of Human Ortholog: 6q22.1

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: protein binding; protein transporter activity

Biological Process: cytokine and chemokine mediated signaling pathway; NLS-bearing substrate import into nucleus