Rabbit anti-Human, Mouse KNG1 Polyclonal Antibody | anti-KNG1 antibody

KNG1 (BDK, KNG, Kininogen-1, Alpha-2-thiol Proteinase Inhibitor, Fitzgerald Factor, High Molecular Weight Kininogen, Williams-Fitzgerald-Flaujeac Factor, Kininogen-1 Heavy Chain, T-kinin, Ile-Ser-Bradykinin, Bradykinin, Kallidin I, Lysyl-bradykinin, Kalli

Cat. Num. AAA6383776

• Gene Names: KNG1; BK; BDK; KNG
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KNG1; Polyclonal Antibody; KNG1 (BDK; KNG; Kininogen-1; Alpha-2-thiol Proteinase Inhibitor; Fitzgerald Factor; High Molecular Weight Kininogen; Williams-Fitzgerald-Flaujeac Factor; Kininogen-1 Heavy Chain; T-kinin; Ile-Ser-Bradykinin; Bradykinin; Kallidin I; Lysyl-bradykinin; Kalli; anti-KNG1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KNG1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-KNG1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human KNG1, aa1-427 (NP_000884.1).
• Immunogen Sequence: MKLITILFLCSRLLLSLTQESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRITYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLGMISLMKRPPGFSPFRSSRIGEIKEETTSHLRSCEYKGRPPKAGAEPASEREVS
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KNG1 rabbit polyclonal antibody. Western Blot analysis of KNG1 expression in mouse spleen.)

Western Blot (WB)

(Western Blot analysis of KNG1 expression in transfected 293T cell line by KNG1 polyclonal antibody. Lane 1: KNG1 transfected lysate (47.9kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-KNG1 antibody

This gene uses alternative splicing to generate two different proteins-high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-KNG1 antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4504893
• NCBI GeneID: 3827
• NCBI Accession #: NP_000884.1
• NCBI GenBank Nucleotide #: NM_000893
• UniProt Accession #: P01042; P01043; Q53EQ0; Q6PAU9; Q7M4P1; A8K474; B2RCR2
• Molecular Weight: 71,957 Da
• NCBI Official Full Name: kininogen-1 isoform 2
• NCBI Official Synonym Full Names: kininogen 1
• NCBI Official Symbol: KNG1
• NCBI Official Synonym Symbols: BK; BDK; KNG
• NCBI Protein Information: kininogen-1; HMWK; bradykinin; fitzgerald factor; high molecular weight kininogen; alpha-2-thiol proteinase inhibitor; williams-Fitzgerald-Flaujeac factor
• UniProt Protein Name: Kininogen-1
• Protein Family: Kininogen
• UniProt Gene Name: KNG1
• UniProt Synonym Gene Names: BDK; KNG; HMWK
• UniProt Entry Name: KNG1_HUMAN

NCBI Description

This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

Uniprot Description

KNG1: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency). HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted; Inhibitor; Secreted, signal peptide; Contractile; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: heparin binding; protein binding; zinc ion binding; cysteine protease inhibitor activity; receptor binding

Biological Process: negative regulation of proteolysis; platelet activation; elevation of cytosolic calcium ion concentration; smooth muscle contraction; platelet degranulation; positive regulation of apoptosis; negative regulation of blood coagulation; negative regulation of cell adhesion; blood coagulation; inflammatory response; vasodilation; blood coagulation, intrinsic pathway

Disease: High Molecular Weight Kininogen Deficiency