Rabbit anti-Human KLRC1 Polyclonal Antibody | anti-KLRC1 antibody

KLRC1, CT (Killer Cell Lectin-like Receptor Subfamily C Member 1, CD159 Antigen-like Family Member A, CD159a, MGC13374, MGC59791, NK Cell Receptor A, NKG2A, NKG2, NKG2-A/B-activating NK Receptor, NKG2-A/NKG2-B Type II Integral Membrane Protein) (PE)

Cat. Num. AAA6484524

• Gene Names: KLRC1; NKG2; NKG2A; CD159A
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: KLRC1; Polyclonal Antibody; CT (Killer Cell Lectin-like Receptor Subfamily C Member 1; CD159 Antigen-like Family Member A; CD159a; MGC13374; MGC59791; NK Cell Receptor A; NKG2A; NKG2; NKG2-A/B-activating NK Receptor; NKG2-A/NKG2-B Type II Integral Membrane Protein) (PE); anti-KLRC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KLRC1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-KLRC1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-term region of human KLRC1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KLRC1 Antibody (C-term) in Jurkat cell line lysates (35ug/lane). KLRC1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human kidney carcinoma with KLRC1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of jurkat cells using KLRC1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of KLRC1 Antibody (C-term) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-KLRC1 antibody

Antibodies; Receptors

References

1. Brooks, A.G., et.al., J. Exp. Med. 185(4), 795-800 (1997). 2. Plougastel, B., et.al., Immunogenetics 44(4), 286-291 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4504881
• NCBI GeneID: 3821
• NCBI Accession #: NP_002250.1
• NCBI GenBank Nucleotide #: NM_002259.4
• UniProt Accession #: P26715
• Molecular Weight: 26,314 Da
• NCBI Official Full Name: NKG2-A/NKG2-B type II integral membrane protein isoform NKG2-A
• NCBI Official Synonym Full Names: killer cell lectin-like receptor subfamily C, member 1
• NCBI Official Symbol: KLRC1
• NCBI Official Synonym Symbols: NKG2; NKG2A; CD159A
• NCBI Protein Information: NKG2-A/NKG2-B type II integral membrane protein; NK cell receptor A; C-lectin type II protein; natural killer cell lectin; natural killer group protein 2; NKG2-1/B activating NK receptor; NKG2-A/B-activating NK receptor; CD159 antigen-like family member A
• UniProt Protein Name: NKG2-A/NKG2-B type II integral membrane protein
• UniProt Gene Name: KLRC1
• UniProt Synonym Gene Names: NKG2A
• UniProt Entry Name: NKG2A_HUMAN

NCBI Description

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. The protein encoded by this gene belongs to the killer cell lectin-like receptor family, also called NKG2 family, which is a group of transmembrane proteins preferentially expressed in NK cells. This family of proteins is characterized by the type II membrane orientation and the presence of a C-type lectin domain. This protein forms a complex with another family member, KLRD1/CD94, and has been implicated in the recognition of the MHC class I HLA-E molecules in NK cells. The genes of NKG2 family members form a killer cell lectin-like receptor gene cluster on chromosome 12. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

NKG2A: Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: protein binding; transmembrane receptor activity; carbohydrate binding

Biological Process: regulation of immune response; cell surface receptor linked signal transduction