Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Goat Klotho Polyclonal Antibody | anti-KL antibody
Goat anti-Klotho Antibody
Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining in luminal epithelial cells of ducts, while Human Placenta shows membranous staining. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments in human brain, duodenum, placenta and prostate lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]
Uniprot Description
Klotho: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1. Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.2.1.31; Hydrolase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 13q12
Cellular Component: apical plasma membrane; extracellular region; extracellular space; integral to membrane; integral to plasma membrane; intracellular; plasma membrane
Molecular Function: 1-phosphatidylinositol-3-kinase activity; beta-glucosidase activity; beta-glucuronidase activity; fibroblast growth factor binding; fibroblast growth factor receptor binding; hormone activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; Ras guanyl-nucleotide exchange factor activity; signal transducer activity; vitamin D binding
Biological Process: aging; calcium ion homeostasis; carbohydrate metabolic process; energy reserve metabolic process; fibroblast growth factor receptor signaling pathway; insulin receptor signaling pathway; MAPKKK cascade; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; positive regulation of bone mineralization; positive regulation of GTPase activity; regulation of phosphoinositide 3-kinase cascade
Disease: Tumoral Calcinosis, Hyperphosphatemic, Familial
Research Articles on KL
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Product Notes
The KL kl (Catalog #AAA421452) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Klotho Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Klotho can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 4000. Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining in luminal epithelial cells of ducts, while Human Placenta shows membranous staining. Recommended concentration, 4-6ug/ml. Western Blot: Preliminary experiments in human brain, duodenum, placenta and prostate lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). Researchers should empirically determine the suitability of the KL kl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KRDDAKYMYY LKK. It is sometimes possible for the material contained within the vial of "Klotho, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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