Rabbit anti-Human, Mouse KLHL9 Polyclonal Antibody | anti-KLHL9 antibody

KLHL9, ID (KLHL9, KIAA1354, Kelch-like protein 9) (Azide free) (HRP)

Cat. Num. AAA6314253

• Gene Names: KLHL9; RP11-380P16.6
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KLHL9; Polyclonal Antibody; ID (KLHL9; KIAA1354; Kelch-like protein 9) (Azide free) (HRP); Kelch-like protein 9; anti-KLHL9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-KLHL9 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLHL9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307-337 amino acids from the Central region of human KLHL9.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KLHL9 Antibody (Center) in mouse testis tissue lysates (35ug/lane).KLHL9 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(KLHL9 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KLHL9 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(KLHL9 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KLHL9 antibody

KLHL9 is substrate-specific adapter for a CUL3-based E3 ubiquitin-protein ligase complex. Within this complex, it controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.

Product Categories/Family for anti-KLHL9 antibody

Antibodies; KIAA; orf

NCBI and Uniprot Product Information

• NCBI GI #: 24308181
• NCBI GeneID: 55958
• NCBI Accession #: NP_061335.1
• NCBI GenBank Nucleotide #: NM_018847.2
• UniProt Accession #: Q9P2J3; Q8TCQ2
• Molecular Weight: 69,429 Da
• NCBI Official Full Name: kelch-like protein 9
• NCBI Official Synonym Full Names: kelch-like family member 9
• NCBI Official Symbol: KLHL9
• NCBI Official Synonym Symbols: RP11-380P16.6
• NCBI Protein Information: kelch-like protein 9; kelch-like 9
• UniProt Protein Name: Kelch-like protein 9
• Protein Family: Kelch-like protein
• UniProt Gene Name: KLHL9
• UniProt Synonym Gene Names: KIAA1354
• UniProt Entry Name: KLHL9_HUMAN

Uniprot Description

KLHL9: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.

Protein type: Ubiquitin conjugating system; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9p22

Cellular Component: midbody

Molecular Function: ubiquitin-protein ligase activity

Biological Process: mitosis; cytokinesis; protein ubiquitination