Rabbit KLHL12 Polyclonal Antibody | anti-KLHL12 antibody

KLHL12 Antibody

Cat. Num. AAA9630755

• Gene Names: KLHL12; DKIR; C3IP1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: KLHL12; Polyclonal Antibody; KLHL12 Antibody; C3IP1; CUL3 interacting protein 1; CUL3-interacting protein 1; DKIR; DKIR homolog; FLJ27152; hDKIR; Kelch like 12 (Drosophila); Kelch like 12; Kelch like 12 variant; Kelch like protein 12; Kelch like protein C3IP1; kelch-like family member 12; Kelch-like protein 12; KLH12_HUMAN; KLHL 12; klhl12; OTTHUMP00000038799; anti-KLHL12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: KLHL12 Antibody detects endogenous levels of KLHL12.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-KLHL12 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human KLHL12.
• Conjugation: Unconjugated
• Similarity: The BTB domain is required for interaction with CUL3.
• Post Translational Modifications: Ubiquitinated by the SCF(FBXL17) complex, leading to its degradation by the proteaseome: ubiquitination by the SCF(FBXL17) complex takes place when aberrant BTB domain dimers are formed.
• Subunit Structure: Component of the BCR(KLHL12) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL12 and RBX1. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A. Interacts with DRD4, KLHL2 and SEC31A. Interacts with PEF1 and PDCD6/ALG-2; interaction takes place in response to cytosolic calcium increase and leads to bridge together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B).
• Tissue Specificity: Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland.
• Function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). The BCR(KLHL12) complex is also involved in neural crest specification: in response to cytosolic calcium increase, interacts with the heterodimer formed with PEF1 and PDCD6/ALG-2, leading to bridge together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export. As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4 and PEF1, without leading to degradation of these proteins.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-KLHL12 antibody

Cytoplasmic vesicle; COPII-coated vesicle; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 97054498
• NCBI GeneID: 59349
• UniProt Accession #: Q53G59; Q9HBX5; A6NEN8; B7Z7B8
• Molecular Weight: 568
• NCBI Official Full Name: Kelch-like protein 12
• NCBI Official Synonym Full Names: kelch-like family member 12
• NCBI Official Symbol: KLHL12
• NCBI Official Synonym Symbols: DKIR; C3IP1
• NCBI Protein Information: kelch-like protein 12; DKIR homolog; kelch-like protein C3IP1; CUL3-interacting protein 1
• UniProt Protein Name: Kelch-like protein 12
• Protein Family: Kelch-like protein
• UniProt Gene Name: KLHL12
• UniProt Synonym Gene Names: C3IP1; hDKIR
• UniProt Entry Name: KLH12_HUMAN

NCBI Description

This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

KLHL12: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4. Component of the BCR(KLHL12) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL12 and RBX1. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A. Interacts with DRD4, KLHL12 and SEC31A. Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: Golgi membrane; ER to Golgi transport vesicle; cytosol

Molecular Function: protein binding

Biological Process: COPII coating of Golgi vesicle; protein monoubiquitination; ER to Golgi vesicle-mediated transport; Wnt receptor signaling pathway