Immunohistochemistry (IHC)
(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Rabbit KLF5 Polyclonal Antibody | anti-KLF5 antibody
Phospho-KLF5 (Ser303) Antibody
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
IHC: 1:50-1:200
ELISA (Peptide): 1:20000-1:40000
Immunohistochemistry (IHC)
(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Immunohistochemistry (IHC)
(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Western Blot (WB)
(Western blot analysis of Phospho-KLF5(Ser303) in lysates of A549, using Phospho-KLF5(Ser303) Antibody.)
Western Blot (WB)
(Western blot analysis of Phospho-KLF5(Ser303) in lysates of A549, using Phospho-KLF5(Ser303) Antibody.)
Post Translational Modifications: Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
Subunit Structure: Interacts with WWP1.
Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family.
NCBI and Uniprot Product Information
Predicted: 51 kDa
NCBI Description
This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
Uniprot Description
KLF5: Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.
Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 13q22.1
Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; cytoplasm; plasma membrane
Molecular Function: protein binding; metal ion binding
Biological Process: transcription from RNA polymerase II promoter; microvillus biogenesis; positive regulation of cell proliferation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; negative regulation of transcription from RNA polymerase II promoter; regulation of microvillus biogenesis
Research Articles on KLF5
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Product Notes
The KLF5 klf5 (Catalog #AAA9610404) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-KLF5 (Ser303) Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's KLF5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA (Peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the KLF5 klf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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