Western Blot (WB)
(Anti-KLF3 antibody, MBS176096, Western blottingLane 1: Rat Testis Tissue LysateLane 2: HELA Cell Lysate )
Rabbit KLF3 Polyclonal Antibody | anti-KLF3 antibody
Anti-KLF3 antibody
Gene Names
KLF3; BKLF
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
KLF3; Polyclonal Antibody; Anti-KLF3 antibody; Krueppel-like factor 3; Kruppel-like factor 3(basic); Basic krueppel-like factor antibody; Basic Kruppel like factor antibody; BKLF3 antibody; CACCC box binding protein BKLF antibody; CACCC-box-binding protein BKLF antibody; Klf3 antibody; KLF3_HUMAN antibody; Krueppel-like factor 3 antibody; Kruppel like factor 3(basic) antibody; MGC48279 antibody; TEF-2 antibody; TEF2 antibody; Transcript ch138 antibody; Zinc finger protein 741 antibody; ZNF741 antibody; anti-KLF3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
345
Applicable Applications for anti-KLF3 antibody
Western Blot (WB)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human KLF3 (20-34aa SYPSNYMESMKPNKY), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-KLF3 antibody, MBS176096, Western blottingLane 1: Rat Testis Tissue LysateLane 2: HELA Cell Lysate )
Western Blot (WB)
(Anti-KLF3 antibody, MBS176096, Western blottingLane 1: Rat Testis Tissue LysateLane 2: HELA Cell Lysate )
Related Product Information for anti-KLF3 antibody
Description: Rabbit IgG polyclonal antibody for Krueppel-like factor 3(KLF3) detection. Tested with WB in Human, Mouse, Rat.
Background: KLF3(Krueppel-like factor 3) is a protein that in humans is encoded by the KLF3 gene. It is also known as BKLF(Basic kruppel-like factor). KLF3 is a member of the Krüppel-like factors family of transcription factors. KLF3 primarily represses transcription through the transcriptional corepressor C-terminal binding protein(CtBP). Suske et al.(2005) stated that the human KLF3 gene maps to chromosome 4p14, and the mouse Klf3 gene maps to chromosome 5C3.3. Suske et al.(2005) stated that the human and mouse KLF3 proteins contain 345 and 344 amino acids, respectively. Both have a characteristic zinc finger domain, but lack the N-terminal buttonhead box found in related SP transcription factors. The repression mediated through KLF3 is also partially dependent on the sumoylation of its K10 and K197. Within KLF3's C-terminal, which is highly conserved throughout the KLF family, resides three C2H2 zinc fingers that preferentially bind the site NRG GNG NGR.
Background: KLF3(Krueppel-like factor 3) is a protein that in humans is encoded by the KLF3 gene. It is also known as BKLF(Basic kruppel-like factor). KLF3 is a member of the Krüppel-like factors family of transcription factors. KLF3 primarily represses transcription through the transcriptional corepressor C-terminal binding protein(CtBP). Suske et al.(2005) stated that the human KLF3 gene maps to chromosome 4p14, and the mouse Klf3 gene maps to chromosome 5C3.3. Suske et al.(2005) stated that the human and mouse KLF3 proteins contain 345 and 344 amino acids, respectively. Both have a characteristic zinc finger domain, but lack the N-terminal buttonhead box found in related SP transcription factors. The repression mediated through KLF3 is also partially dependent on the sumoylation of its K10 and K197. Within KLF3's C-terminal, which is highly conserved throughout the KLF family, resides three C2H2 zinc fingers that preferentially bind the site NRG GNG NGR.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,430 Da
NCBI Official Full Name
Krueppel-like factor 3
NCBI Official Synonym Full Names
Kruppel-like factor 3 (basic)
NCBI Official Symbol
KLF3
NCBI Official Synonym Symbols
BKLF
NCBI Protein Information
Krueppel-like factor 3; TEF-2; transcript ch138; basic Kruppel-like factor; basic kruppel like factor; basic krueppel-like factor; CACCC-box-binding protein BKLF
UniProt Protein Name
Krueppel-like factor 3
Protein Family
UniProt Gene Name
KLF3
UniProt Synonym Gene Names
BKLF
UniProt Entry Name
KLF3_HUMAN
Uniprot Description
KLF3: Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis. Belongs to the krueppel C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; C2H2-type zinc finger protein; DNA-binding
Chromosomal Location of Human Ortholog: 4p14
Cellular Component: nucleus
Molecular Function: DNA binding; metal ion binding; transcription factor activity
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; multicellular organismal development
Research Articles on KLF3
Similar Products
Product Notes
The KLF3 klf3 (Catalog #AAA176096) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-KLF3 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KLF3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the KLF3 klf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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