Rabbit anti-Human KLF11 Polyclonal Antibody | anti-KLF11 antibody

KLF11, NT (Krueppel-like Factor 11, Transforming Growth Factor-beta-inducible Early Growth Response Protein 2, TGFB-inducible Early Growth Response Protein 2, TIEG-2, TIEG2, FKLF) (MaxLight 650)

Cat. Num. AAA6484489

• Gene Names: KLF11; FKLF; FKLF1; MODY7; TIEG2; Tieg3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: KLF11; Polyclonal Antibody; NT (Krueppel-like Factor 11; Transforming Growth Factor-beta-inducible Early Growth Response Protein 2; TGFB-inducible Early Growth Response Protein 2; TIEG-2; TIEG2; FKLF) (MaxLight 650); anti-KLF11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KLF11.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-KLF11 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human KLF11 (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TIEG2 antibody (N-term) in K562 cell line lysates (35ug/lane). TIEG2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with TIEG2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells using TIEG2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of TIEG2 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-KLF11 antibody

Antibodies; Transcription Factors

References

1. Kuroda, E., Endocr. J. 56(2), 275-286 (2009). 2. Ma, L., J. Clin. Endocrinol. Metab. 93(9), 3644-3649 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4507503
• NCBI GeneID: 8462
• NCBI Accession #: NP_003588
• NCBI GenBank Nucleotide #: NM_003597
• UniProt Accession #: O14901
• Molecular Weight: 55kDa
• NCBI Official Full Name: Krueppel-like factor 11 isoform a
• NCBI Official Synonym Full Names: Kruppel like factor 11
• NCBI Official Symbol: KLF11
• NCBI Official Synonym Symbols: FKLF; FKLF1; MODY7; TIEG2; Tieg3
• NCBI Protein Information: Krueppel-like factor 11
• UniProt Protein Name: Krueppel-like factor 11
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF11
• UniProt Synonym Gene Names: FKLF; TIEG2; TGFB-inducible early growth response protein 2; TIEG-2
• UniProt Entry Name: KLF11_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger transcription factor that binds to SP1-like sequences in epsilon- and gamma-globin gene promoters. This binding inhibits cell growth and causes apoptosis. Defects in this gene are a cause of maturity-onset diabetes of the young type 7 (MODY7). Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Uniprot Description

TIEG2: Transcription factor. Activates the epsilon- and gamma- globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. Represses transcription of SMAD7 which enhances TGF-beta signaling. Induces apoptosis. Defects in KLF11 are the cause of maturity-onset diabetes of the young type 7 (MODY7). MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Belongs to the Sp1 C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: nucleus

Molecular Function: metal ion binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; G1/S-specific transcription in mitotic cell cycle; negative regulation of cell proliferation; positive regulation of apoptosis; apoptosis; negative regulation of transcription from RNA polymerase II promoter

Disease: Maturity-onset Diabetes Of The Young, Type 7