Mouse anti-Mouse Kinase D-interacting substrate 220, Kidins 220 Polyclonal Antibody | anti-KIDINS220 antibody

Anti-Kinase D-interacting substrate 220, Kidins 220a antibody

Cat. Num. AAA355143

• Gene Names: KIDINS220; ARMS
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Protein A or Protein G purified
• Synonyms: Kinase D-interacting substrate 220; Kidins 220; Polyclonal Antibody; Anti-Kinase D-interacting substrate 220; Kidins 220a antibody; Monoclonal Anti- Kinase D-interacting substrate 220; anti-KIDINS220 antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG1
• Clone Number: K23
• Purity/Purification: Protein A or Protein G purified
• Form/Format: Liquid

• Applicable Applications for anti-KIDINS220 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Application Notes: WB (1:500-1000); ICC (1:100-500)
• Immunogen: Recombinant Kidins 220 protein.
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
• Preparation and Storage: Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Western Blot (WB)

Related Product Information for anti-KIDINS220 antibody

Protein kinase D (PKD) is a serine/threonine kinase regulated by diacylglycerol signaling pathways with unique domain composition and enzymatic properties, still awaiting identification of its specific substrate(s). Kinase D-interacting substrate 220(Kidins220) is an enzyme that in humans is encoded by the KIDINS220 gene. [1] It is an integral membrane protein selectively expressed in brain and neuroendocrine cells, where it concentrates at the tip of neurites. and it contains 11 ankyrin repeats and four transmembrane domains within the N-terminal region.

References

1. Iglesias T, Cabrera-Poch N, Mitchell MP, Naven TJ, Rozengurt E, Schiavo G (Jan 2001). "Identification and cloning of Kidins220, a novel neuronal substrate of protein kinase D". J Biol Chem 275 (51): 40048-56.

NCBI and Uniprot Product Information

• NCBI GI #: 55741641
• NCBI GeneID: 57498
• NCBI Accession #: NP_065789.1
• NCBI GenBank Nucleotide #: NM_020738.2
• UniProt Accession #: Q9ULH0; Q4VC08; Q6MZU2; Q9H889; Q9H9E4; Q9NT37; Q9UF42; A1L4N4
• Molecular Weight: 196,542 Da
• NCBI Official Full Name: kinase D-interacting substrate of 220 kDa
• NCBI Official Synonym Full Names: kinase D-interacting substrate, 220kDa
• NCBI Official Symbol: KIDINS220
• NCBI Official Synonym Symbols: ARMS
• NCBI Protein Information: kinase D-interacting substrate of 220 kDa; ankyrin repeat-rich membrane spanning; ankyrin repeat-rich membrane-spanning protein
• UniProt Protein Name: Kinase D-interacting substrate of 220 kDa
• Protein Family: Kinase D-interacting substrate
• UniProt Gene Name: KIDINS220
• UniProt Synonym Gene Names: ARMS; KIAA1250
• UniProt Entry Name: KDIS_HUMAN

Uniprot Description

Function: Promotes a prolonged MAP-kinase signaling by neurotrophins through activation of a Rap1-dependent mechanism. Provides a docking site for the CRKL-C3G complex, resulting in Rap1-dependent sustained ERK activation. May play an important role in regulating postsynaptic signal transduction through the syntrophin-mediated localization of receptor tyrosine kinases such as EPHA4. In cooperation with SNTA1 can enhance EPHA4-induced JAK/STAT activation. May play a role in neurotrophin- and ephrin-mediated neuronal outgrowth and in axon guidance during neural development and in neuronal regeneration

By similarity. Modulates stress-induced apoptosis of melanoma cells via regulation of the MEK/ERK signaling pathway. Ref.6

Subunit structure: Interacts with NTRK1, NTRK2, NTRK3, ERKL and NGFR. Can form a ternary complex with NGFR and NTRK1 and this complex is affected by the expression levels of KIDINS220/ARMS. An increase in KIDINS220/ARMS expression leads to a decreased association of NGFR and NTRK1. Interacts with SNTA1 and SNTB2 and binds to their PDZ domains. Interacts with EPHA4 and PRKD1

By similarity.

Subcellular location: Membrane; Multi-pass membrane protein

Potential.

Tissue specificity: Abundant in developing and adult neural tissues as well as neuroendocrine cells and dendritic cells. Overexpressed in melanoma and melanoma cell lines. Ref.6

Domain: The transmembrane domain mediates interaction with NTRK1

By similarity.

Post-translational modification: Tyrosine phosphorylated by NTRK1, NTRK2, EPHB2 and EPHA4. Phosphorylation at Ser-918 is induced by phorbol ester treatment. Phosphorylation by NTRK2 is induced by brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. Phosphorylation by NTRK1 is induced by nerve growth factor (NGF)

By similarity.

Sequence similarities: Contains 12 ANK repeats.Contains 1 KAP NTPase domain.

Sequence caution: The sequence BAA86564.2 differs from that shown. Reason: Erroneous initiation. The sequence BAB14728.1 differs from that shown. Reason: Erroneous initiation.