Rabbit anti-Human KIF5A Polyclonal Antibody | anti-KIF5A antibody

KIF5A, ID (Kinesin Heavy Chain Isoform 5A, Kinesin Heavy Chain Neuron-specific 1, Neuronal Kinesin Heavy Chain, NKHC, NKHC1)

Cat. Num. AAA644059

• Gene Names: KIF5A; NKHC; MY050; SPG10; D12S1889
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: KIF5A; Polyclonal Antibody; ID (Kinesin Heavy Chain Isoform 5A; Kinesin Heavy Chain Neuron-specific 1; Neuronal Kinesin Heavy Chain; NKHC; NKHC1); Anti -KIF5A; anti-KIF5A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KIF5A. Species sequence homology: mouse and rat
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-KIF5A antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.; Dilution: Western Blot: 0.5-1ug/ml; Immunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml; IOptimal dilutions to be determined by the researcher.
• Immunogen: Synthetic peptide corresponding to 20aa from near the center of human KIF5 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with KIF5A antibody at 5ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Western Blot (WB)

(Western blot analysis of KIF5 in K562 cell lysate with KIF5 antibody at (A) 0.5 and (B) 1ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of KIF5 in K562 cells with KIF5 antibody at 5ug/ml.)

Related Product Information for anti-KIF5A antibody

Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350aa, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.

Product Categories/Family for anti-KIF5A antibody

Antibodies; Abs to Motor Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 45446749
• NCBI GeneID: 3798
• NCBI Accession #: NP_004975.2
• NCBI GenBank Nucleotide #: NM_004984.2
• UniProt Accession #: Q12840; Q4LE26; A6H8M5
• Molecular Weight: 117,378 Da
• NCBI Official Full Name: kinesin heavy chain isoform 5A
• NCBI Official Synonym Full Names: kinesin family member 5A
• NCBI Official Symbol: KIF5A
• NCBI Official Synonym Symbols: NKHC; MY050; SPG10; D12S1889
• NCBI Protein Information: kinesin heavy chain isoform 5A; KIF5A variant protein; neuronal kinesin heavy chain; kinesin heavy chain neuron-specific 1; kinesin, heavy chain, neuron-specific
• UniProt Protein Name: Kinesin heavy chain isoform 5A
• Protein Family: Kinesin
• UniProt Gene Name: KIF5A
• UniProt Synonym Gene Names: NKHC1; NKHC
• UniProt Entry Name: KIF5A_HUMAN

NCBI Description

This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10. [provided by RefSeq, Jul 2008]

Uniprot Description

KIF5A: Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10). An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Belongs to the kinesin-like protein family. Kinesin subfamily.

Protein type: Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: microtubule; kinesin complex; membrane; perinuclear region of cytoplasm; cytosol

Molecular Function: protein binding; plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; motor activity; ATP binding

Biological Process: synaptic transmission; axon guidance; protein localization; metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; cytoskeleton-dependent intracellular transport; blood coagulation; microtubule-based movement

Disease: Spastic Paraplegia 10, Autosomal Dominant