Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using KIF4A antibody at 1:7000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Rabbit KIF4A Polyclonal Antibody | anti-KIF4A antibody
KIF4A Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using KIF4A antibody at 1:7000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using KIF4A antibody at 1:7000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.[provided by RefSeq, Mar 2010]
Uniprot Description
KIF4A: Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. Belongs to the kinesin-like protein family. Chromokinesin subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Microtubule-binding; Motor
Chromosomal Location of Human Ortholog: Xq13.1
Cellular Component: cytoplasm; cytosol; intercellular bridge; kinesin complex; membrane; midbody; nucleoplasm; spindle microtubule
Molecular Function: microtubule motor activity; plus-end-directed microtubule motor activity; protein binding
Biological Process: anterograde axon cargo transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; cytokinesis after mitosis; microtubule-based movement; organelle organization and biogenesis; retrograde vesicle-mediated transport, Golgi to ER; spindle midzone assembly involved in mitosis
Disease: Mental Retardation, X-linked 100
Research Articles on KIF4A
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Product Notes
The KIF4A kif4a (Catalog #AAA9130704) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KIF4A Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KIF4A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 - 1:3000. Researchers should empirically determine the suitability of the KIF4A kif4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIF4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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