Rabbit anti-Human KIF16b Polyclonal Antibody | anti-KIF16b antibody

KIF16b (KIF16B, C20orf23, KIAA1590, SNX23, Kinesin-like protein KIF16B, Sorting nexin-23) (AP)

Cat. Num. AAA6313215

• Gene Names: KIF16B; SNX23; C20orf23; KISC20ORF
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KIF16b; Polyclonal Antibody; KIF16b (KIF16B; C20orf23; KIAA1590; SNX23; Kinesin-like protein KIF16B; Sorting nexin-23) (AP); KIF16B; Sorting nexin-23; anti-KIF16b antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 1317

• Applicable Applications for anti-KIF16b antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KIF16b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected between amino acids 10-40 from human KIF16b.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(KIF16b Antibody western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the KIF16b antibody detected the KIF16b protein (arrow).)

Immunohistochemistry (IHC)

(KIF16b Antibody (AP15010a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KIF16b Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-KIF16b antibody

KIF16b may be involved in several stages of intracellular trafficking. Probable microtubule-dependent motor protein (By similarity).

Product Categories/Family for anti-KIF16b antibody

Antibodies; SNX (Sorting Nexin) Proteins

References

Vasilescu, J., et al. J. Proteome Res. 6(1):298-305(2007). Seet, L.F., et al. Biochim. Biophys. Acta 1761(8):878-896(2006). Miki, H., et al. Trends Cell Biol. 15(9):467-476(2005). Hoepfner, S., et al. Cell 121(3):437-450(2005). Worby, C.A., et al. Nat. Rev. Mol. Cell Biol. 3(12):919-931(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 41327691
• NCBI GeneID: 55614
• NCBI Accession #: NP_078980.3
• NCBI GenBank Nucleotide #: NP_078980.3
• UniProt Accession #: Q96L93
• NCBI Official Full Name: kinesin-like protein KIF16B isoform 2
• NCBI Official Synonym Full Names: kinesin family member 16B
• NCBI Official Symbol: KIF16B
• NCBI Official Synonym Symbols: SNX23; C20orf23; KISC20ORF
• NCBI Protein Information: kinesin-like protein KIF16B
• UniProt Protein Name: Kinesin-like protein KIF16B
• Protein Family: Kinesin-like protein
• UniProt Gene Name: KIF16B
• UniProt Synonym Gene Names: C20orf23; KIAA1590; SNX23
• UniProt Entry Name: KI16B_HUMAN

NCBI Description

The protein encoded by this gene is a kinesin-like protein that may be involved in intracellular trafficking. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

Function: Plus end-directed microtubule-dependent motor protein involved in endosome transport and receptor recycling and degradation. Regulates the plus end motility of early endosomes and the balance between recycling and degradation of receptors such as EGF receptor (EGFR) and FGF receptor (FGFR). Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. Ref.9

Subunit structure: Interacts with RAB14

By similarity. Interacts with PTPN21. Ref.11

Subcellular location: Cytoplasm › cytoskeleton

Probable. Early endosome membrane. Note: It is unclear whether association with endosomes is mediated via phosphatidylinositol 3-phosphate (PtdIns3P)-binding or via its interaction with RAB14. Ref.9 Ref.13

Tissue specificity: Primarily expressed in brain. Also present in kidney, liver, intestine, placenta, leukocytes, heart and skeletal muscle (at protein level). Ref.9

Domain: The PX domain mediates binding to phosphatidylinositol 3-phosphate (PtdIns3P), phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2), phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3). Does not bind phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Ref.9 Ref.13

Post-translational modification: Isoform 4 is ubiquitinated at Lys-685.

Sequence similarities: Belongs to the kinesin-like protein family.Contains 1 FHA domain.Contains 1 kinesin-motor domain.Contains 1 PX (phox homology) domain.

Sequence caution: The sequence BAA90971.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAA90971.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.The sequence BAB13416.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence BAB15530.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAI43062.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI43179.1 differs from that shown. Reason: Erroneous gene model prediction.