Rabbit anti-Human KIAA1324 / maba1 Polyclonal Antibody | anti-KIAA1324 antibody

Rabbit Polyclonal to Human KIAA1324 / maba1

Cat. Num. AAA244835

• Gene Names: KIAA1324; EIG121
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Peptide column affinity chromatography
• Synonyms: KIAA1324 / maba1; Polyclonal Antibody; Rabbit Polyclonal to Human KIAA1324 / maba1; Anti-KIAA1324 / maba1 Antibody (C-Terminus) IHC-plus; KIAA1324; EIG121; Estrogen induced gene 121; Maba1; RP11-352P4.1; UPF0577 protein KIAA1324; Human KIAA1324; maba1; anti-KIAA1324 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least for isoforms of EIG121 known to exist. EIG121 antibody is predicted to not cross-react with other UPF 0577 protein family members.
• Purity/Purification: Peptide column affinity chromatography
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1013

• Applicable Applications for anti-KIAA1324 antibody: Immunohistochemistry (IHC), Immunohistochemistry (IHC-P); Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC-P: 5ug/ul; WB: 1-2ug/ml; ; Optimal dilution to be determined by the researcher.; ; IG121 antibody can be used for detection of EIG121 by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Immunogen Species: KIAAA1324/ maba1 antibody was raised against human.
• Antigen Type: Synthetic peptide
• Immunogen: KIA1324/maba1 antibody was raised against an 18 amino acids synthetic peptide near the C-Terminus of human EIG121. The immunogen is located within the last 50 amino acids of EIG121.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-KIAA1324 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of EIG121 in MCF7 cells with EIG121 antibody at 20 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of EIG121 in MCF7 cells with EIG121 antibody at 5 ug/ml.)

Western Blot (WB)

(Western blot analysis of EIG121 in MCF7 cell lysate with EIG121 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 38569482
• NCBI GeneID: 57535
• NCBI Accession #: NP_065826.2
• NCBI GenBank Nucleotide #: NM_020775.4
• UniProt Accession #: Q6UXG2; Q08AE6; Q5T5C9; Q5T5D0; Q5T5D1; Q9P2M2
• NCBI Official Full Name: UPF0577 protein KIAA1324 isoform 1
• NCBI Official Synonym Full Names: KIAA1324
• NCBI Official Symbol: KIAA1324
• NCBI Official Synonym Symbols: EIG121
• NCBI Protein Information: UPF0577 protein KIAA1324; maba1; estrogen-induced gene 121 protein
• UniProt Protein Name: UPF0577 protein KIAA1324
• Protein Family: UPF0577 protein
• UniProt Gene Name: KIAA1324
• UniProt Synonym Gene Names: EIG121
• UniProt Entry Name: K1324_HUMAN

NCBI Description

Expression of this gene is induced by estrogen and the encoded protein has been characterized as a transmembrane protein. The encoded protein has been found in to correlate with survival in certain carcinomas (PMID: 21102415) and may be important for cellular response to stress (PMID: 21072319). Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2012]

Uniprot Description

KIAA1324: May play a role as a marker of hyperestrogenic state and estrogen-related type I endometrial carcinoma. Belongs to the UPF0577 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: integral to plasma membrane; lysosomal membrane; lysosome; late endosome membrane; late endosome; trans-Golgi network

Biological Process: macroautophagy; cellular response to starvation