Western Blot (WB)
(Western Blot: Sample: Rat Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat KRT2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit Keratin 2 (KRT2) Polyclonal Antibody | anti-KRT2 antibody
Polyclonal Antibody to Keratin 2 (KRT2)
Gene Names
Krt2; Kb2; CK 2e
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Keratin 2 (KRT2); Polyclonal Antibody; Polyclonal Antibody to Keratin 2 (KRT2); anti-KRT2 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against KRT2. It has been selected for its ability to recognize KRT2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-ECR MSGDFSDNVS VSVTSSTISS SVASKAGFGS GGQSSGGRGS YGGRGGGSTY GSGGRSSGVR GSGSGSGGGG YSSGGGSRGG SGGGGYSTGG GSRGGSSSGG GGYSSGGGSR GDSSSGGGSR GGSGGGSRGG SGGGGYSSGG GSRGGSSSGG AVSGSERGGS GSGEGCGSGV TFSFR
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-ECR MSGDFSDNVS VSVTSSTISS SVASKAGFGS GGQSSGGRGS YGGRGGGSTY GSGGRSSGVR GSGSGSGGGG YSSGGGSRGG SGGGGYSTGG GSRGGSSSGG GGYSSGGGSR GDSSSGGGSR GGSGGGSRGG SGGGGYSSGG GSRGGSSSGG AVSGSERGGS GSGEGCGSGV TFSFR
Applicable Applications for anti-KRT2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant KRT2 (Glu508~Arg685) expressed in E.coli.
Cross Reactivity
Human, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2043053
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat KRT2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat KRT2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Rat Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69,127 Da
NCBI Official Full Name
keratin, type II cytoskeletal 2 epidermal
NCBI Official Synonym Full Names
keratin 2
NCBI Official Symbol
Krt2
NCBI Official Synonym Symbols
Kb2; CK 2e
NCBI Protein Information
keratin, type II cytoskeletal 2 epidermal
UniProt Protein Name
Keratin, type II cytoskeletal 2 epidermal
Protein Family
UniProt Gene Name
Krt2
UniProt Synonym Gene Names
CK-2e; K2e
Uniprot Description
K2: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 7q36
Cellular Component: cornified envelope; extracellular space; keratin filament; membrane; nucleus
Molecular Function: cytoskeletal protein binding; structural constituent of epidermis; structural molecule activity
Biological Process: epidermis development; intermediate filament organization; keratinization; keratinocyte activation; keratinocyte migration; keratinocyte proliferation; peptide cross-linking
Similar Products
Product Notes
The KRT2 krt2 (Catalog #AAA2007134) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Keratin 2 (KRT2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 2 (KRT2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the KRT2 krt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-ECR MSGDFSDNVS VSVTSSTISS SVASKAGFGS GGQSSGGRGS YGGRGGGSTY GSGGRSSGVR GSGSGSGGGG YSSGGGSRGG SGGGGYSTGG GSRGGSSSGG GGYSSGGGSR GDSSSGGGSR GGSGGGSRGG SGGGGYSSGG GSRGGSSSGG AVSGSERGGS GSGEGCGSGV TFSFR. It is sometimes possible for the material contained within the vial of "Keratin 2 (KRT2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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