Western Blot (WB)
(Western Blot: Sample: Rat Ovary lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat KRT19 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit Keratin 19 (KRT19) Polyclonal Antibody | anti-KRT19 antibody
Polyclonal Antibody to Keratin 19 (KRT19)
Gene Names
Krt19; Ka19; Krt1-19
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Keratin 19 (KRT19); Polyclonal Antibody; Polyclonal Antibody to Keratin 19 (KRT19); anti-KRT19 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against KRT19. It has been selected for its ability to recognize KRT19 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- EKITMQNL NDRLASYLDK VRALEQANGE LEVKIRDWYQ KQGPGPFRDY SQYFKTIEDL RDKILGATIE NSKIVLQIDN ARLAADDFRT KFETEQALRM SVEADINGLR RVLDELTLAR TDLEMQIENL KEELAYLKKN HEEEISALRS QVGGQVSVEV DSTPGIDLAK ILSEMRSQYE AMAEKNRKDA EAWYLTQIDE LNTQVAVHTT QIQINKTEVT ELRRKVQDLE IELQSQLSMK AALEGTVAEI EARYGAQLSH IQGVISSIEV QLSNVRADTE RQNQEYQQLM DIKSRLEQEI ATYRSLLEGQ
MGHHHHHHSGSEF- EKITMQNL NDRLASYLDK VRALEQANGE LEVKIRDWYQ KQGPGPFRDY SQYFKTIEDL RDKILGATIE NSKIVLQIDN ARLAADDFRT KFETEQALRM SVEADINGLR RVLDELTLAR TDLEMQIENL KEELAYLKKN HEEEISALRS QVGGQVSVEV DSTPGIDLAK ILSEMRSQYE AMAEKNRKDA EAWYLTQIDE LNTQVAVHTT QIQINKTEVT ELRRKVQDLE IELQSQLSMK AALEGTVAEI EARYGAQLSH IQGVISSIEV QLSNVRADTE RQNQEYQQLM DIKSRLEQEI ATYRSLLEGQ
Sequence Length
21
Applicable Applications for anti-KRT19 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant KRT19 (Glu83~Gln390) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2052986
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Ovary lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat KRT19 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Ovary lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat KRT19 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Ovary Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Ovary Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,636 Da
NCBI Official Full Name
keratin, type I cytoskeletal 19
NCBI Official Synonym Full Names
keratin 19
NCBI Official Symbol
Krt19
NCBI Official Synonym Symbols
Ka19; Krt1-19
NCBI Protein Information
keratin, type I cytoskeletal 19
UniProt Protein Name
Keratin, type I cytoskeletal 19
Protein Family
UniProt Gene Name
Krt19
UniProt Synonym Gene Names
Ka19; Krt1-19; CK-19; K19
Uniprot Description
K19: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 19 differs from all other IF proteins in lacking the C-terminal tail domain. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 10q31
Cellular Component: apicolateral plasma membrane; cell periphery; costamere; dystrophin-associated glycoprotein complex; extracellular exosome; intermediate filament; plasma membrane; sarcolemma; terminal web; Z disc
Molecular Function: protein binding; protein complex binding; structural constituent of muscle
Biological Process: Notch signaling pathway; response to estrogen; sarcomere organization
Research Articles on KRT19
Similar Products
Product Notes
The KRT19 krt19 (Catalog #AAA2005517) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Keratin 19 (KRT19) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 19 (KRT19) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the KRT19 krt19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- EKITMQNL NDRLASYLDK VRALEQANGE LEVKIRDWYQ KQGPGPFRDY SQYFKTIEDL RDKILGATIE NSKIVLQIDN ARLAADDFRT KFETEQALRM SVEADINGLR RVLDELTLAR TDLEMQIENL KEELAYLKKN HEEEISALRS QVGGQVSVEV DSTPGIDLAK ILSEMRSQYE AMAEKNRKDA EAWYLTQIDE LNTQVAVHTT QIQINKTEVT ELRRKVQDLE IELQSQLSMK AALEGTVAEI EARYGAQLSH IQGVISSIEV QLSNVRADTE RQNQEYQQLM DIKSRLEQEI ATYRSLLEGQ. It is sometimes possible for the material contained within the vial of "Keratin 19 (KRT19), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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