Western Blot (WB)
(Western blot analysis of extracts from COS7 cells using Keratin 15 antibody)
Rabbit Keratin 15 Polyclonal Antibody | anti-KRT15 antibody
Keratin 15 antibody
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
Keratin 15; Polyclonal Antibody; Keratin 15 antibody; Polyclonal Keratin 15; Anti-Keratin 15; Keratin type I cytoskeletal 15; Cytokeratin-15; CK-15; K15; anti-KRT15 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Keratin 15 antibody detects endogenous levels of total Keratin 15 protein
Form/Format
Purified IgG in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
456
Applicable Applications for anti-KRT15 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:20000
IHC: 1:50-1:100
ELISA: 1:20000
Biological Significance
Keratin 15 is a type I keratin without a defined type II partner whose expression in epidermal diseases has not been investigated.
Cross-Reactivity
Mouse, Rat
Immunogen
Keratin 15 antibody was raised in Rabbit using a synthesized peptide derived from human Keratin 15 as the immunogen
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeated freeze-thaw cycles
Western Blot (WB)
(Western blot analysis of extracts from COS7 cells using Keratin 15 antibody)
Western Blot (WB)
(Western blot analysis of extracts from COS7 cells using Keratin 15 antibody)
Related Product Information for anti-KRT15 antibody
Rabbit polyclonal Keratin 15 antibody
Product Categories/Family for anti-KRT15 antibody
NCBI and Uniprot Product Information
Uniprot Description
K15: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21.2
Cellular Component: intermediate filament; nucleus
Molecular Function: protein binding; structural constituent of cytoskeleton
Biological Process: epidermis development
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Product Notes
The Keratin 15 krt15 (Catalog #AAA5300359) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Keratin 15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:1000 IHC: 1:50-1:100 ELISA: 1:20000. Researchers should empirically determine the suitability of the Keratin 15 krt15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin 15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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