Rabbit KCNQ3 Polyclonal Antibody | anti-KCNQ3 antibody

KCNQ3 Antibody

Cat. Num. AAA9612500

• Gene Names: KCNQ3; EBN2; BFNC2; KV7.3
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: KCNQ3; Polyclonal Antibody; KCNQ3 Antibody; BFNC 2; BFNC; BFNC2; EBN 2; EBN2; KCNQ 3; KCNQ3_HUMAN; KQT like 3; KQT-like 3; KV7.3; Potassium channel subunit alpha KvLQT3; Potassium channel voltage gated subfamily Q member 3; Potassium voltage gated channel KQT like protein 3; Potassium voltage gated channel KQT like subfamily member 3; Potassium voltage gated channel subfamily KQT member 3; Potassium voltage-gated channel subfamily KQT member 3; Voltage gated potassium channel subunit Kv7.3; Voltage-gated potassium channel subunit Kv7.3; anti-KCNQ3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KCNQ3 Antibody detects endogenous levels of total KCNQ3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 752

• Applicable Applications for anti-KCNQ3 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human KCNQ3, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Mouse heart, using KCNQ3 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-KCNQ3 antibody

Function: Associates with KCNQ2 or KCNQ5 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. Therefore, it is important in the regulation of neuronal excitability.

Post Translational Modifications: KCNQ2/KCNQ3 are ubiquitinated by NEDD4L. Ubiquitination leads to protein degradation (Probable). Degradation induced by NEDD4L is inhibited by USP36 (PubMed:27445338).

Subcellular Location: Cell membrane>Multi-pass membrane protein.

Tissue Specificity: Predominantly expressed in brain.

Subunit Structure: Heterotetramer with KCNQ2; form the heterotetrameric M potassium channel (PubMed:27564677). Interacts with calmodulin; the interaction is calcium-independent, constitutive and participates to the proper assembly of a functional heterotetrameric M channel (PubMed:27564677). Heteromultimer with KCNQ5 (PubMed:11159685). May associate with KCNE2 (PubMed:11034315). Interacts with IQCJ-SCHIP1 (By similarity).

Similarity: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.3/KCNQ3 sub-subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 325651930
• NCBI GeneID: 3786
• NCBI Accession #: NP_001191753.1
• NCBI GenBank Nucleotide #: NP_001191753.1
• UniProt Accession #: O43525
• Molecular Weight: Observed Molecular Weight: (Observed)96kD.; Predicted Molecular Weight: (Calculated)97kDa.
• NCBI Official Full Name: potassium voltage-gated channel subfamily KQT member 3 isoform 2
• NCBI Official Synonym Full Names: potassium voltage-gated channel subfamily Q member 3
• NCBI Official Symbol: KCNQ3
• NCBI Official Synonym Symbols: EBN2; BFNC2; KV7.3
• NCBI Protein Information: potassium voltage-gated channel subfamily KQT member 3
• UniProt Protein Name: Potassium voltage-gated channel subfamily KQT member 3
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: KCNQ3

NCBI Description

This gene encodes a protein that functions in the regulation of neuronal excitability. The encoded protein forms an M-channel by associating with the products of the related KCNQ2 or KCNQ5 genes, which both encode integral membrane proteins. M-channel currents are inhibited by M1 muscarinic acetylcholine receptors and are activated by retigabine, a novel anti-convulsant drug. Defects in this gene are a cause of benign familial neonatal convulsions type 2 (BFNC2), also known as epilepsy, benign neonatal type 2 (EBN2). Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

Kv7.3: Probably important in the regulation of neuronal excitability. Associates with KCNQ2 or KCNQ5 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. Defects in KCNQ3 are the cause of benign familial neonatal seizures type 2 (BFNS2). A disorder characterized by clusters of seizures occurring in the first days of life. Most patients have spontaneous remission by 12 months of age and show normal psychomotor development. The disorder is distinguished from benign familial infantile seizures by an earlier age at onset. Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.3/KCNQ3 sub-subfamily.

Protein type: Channel, potassium; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 8q24.22

Cellular Component: cell surface; integral component of membrane; integral component of plasma membrane; plasma membrane; voltage-gated potassium channel complex

Molecular Function: calmodulin binding; protein binding; voltage-gated potassium channel activity

Biological Process: membrane hyperpolarization; potassium ion transmembrane transport; regulation of ion transmembrane transport; synaptic transmission

Disease: Seizures, Benign Familial Neonatal, 2