Western Blot (WB)
(Anti-KCNJ10 Antibody (Center)at 1:2000 dilution + rat brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit KCNJ10 Polyclonal Antibody | anti-KCNJ10 antibody
KCNJ10 Antibody (Center)
Western Blot (WB)
(Anti-KCNJ10 Antibody (Center)at 1:2000 dilution + rat brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-KCNJ10 Antibody (Center)at 1:2000 dilution + rat brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 43 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(KCNJ10 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the KCNJ10 antibody detected the KCNJ10 protein (arrow).)
Western Blot (WB)
(KCNJ10 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the KCNJ10 antibody detected the KCNJ10 protein (arrow).)
potassium channel family, characterized by having a greater
tendency to allow potassium to flow into, rather than out of, a
cell. The encoded protein may form a heterodimer with another
potassium channel protein and may be responsible for the potassium
buffering action of glial cells in the brain. Mutations in this
gene have been associated with seizure susceptibility of common
idiopathic generalized epilepsy syndromes.
Reichold, M., et al. Proc. Natl. Acad. Sci. U.S.A. 107(32):14490-14495(2010)
Heuser, K., et al. Epilepsy Res. 88(1):55-64(2010)
Pawelczyk, M., et al. Ann. Hum. Genet. 73 (PT 4), 411-421 (2009) :
Yang, T., et al. Am. J. Hum. Genet. 84(5):651-657(2009)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes. [provided by RefSeq, Jul 2008]
Uniprot Description
Kir4.1: May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Defects in KCNJ10 are the cause of seizures, sensorineural deafness, ataxia, mental retardation, and electrolyte imbalance (SESAMES). A complex disorder characterized by generalized seizures with onset in infancy, delayed psychomotor development, ataxia, sensorineural hearing loss, hypokalemia, metabolic alkalosis, and hypomagnesemia. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, potassium; Channel, ligand-gated
Chromosomal Location of Human Ortholog: 1q23.2
Cellular Component: microvillus; basolateral plasma membrane; integral to plasma membrane; apical plasma membrane; plasma membrane
Molecular Function: identical protein binding; protein binding; ATP-activated inward rectifier potassium channel activity; ATP binding; receptor binding
Biological Process: regulation of long-term neuronal synaptic plasticity; myelination in the central nervous system; membrane hyperpolarization; response to glucocorticoid stimulus; response to blue light; glutamate uptake during transmission of nerve impulse; response to mineralocorticoid stimulus; adult walking behavior; protein homotetramerization; synaptic transmission; visual perception; potassium ion import; optic nerve development; regulation of resting membrane potential; regulation of sensory perception of pain; inflammatory response; potassium ion homeostasis; potassium ion transport
Disease: Pendred Syndrome; Seizures, Sensorineural Deafness, Ataxia, Mental Retardation, And Electrolyte Imbalance; Deafness, Autosomal Recessive 4, With Enlarged Vestibular Aqueduct
Research Articles on KCNJ10
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Product Notes
The KCNJ10 kcnj10 (Catalog #AAA9213730) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 203-230. The KCNJ10 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's KCNJ10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the KCNJ10 kcnj10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KCNJ10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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