Rabbit anti-Human KCNH4 Polyclonal Antibody | anti-KCNH4 antibody

KCNH4, NT (KCNH4, Potassium voltage-gated channel subfamily H member 4, Brain-specific eag-like channel 2, Ether-a-go-go-like potassium channel 1, Voltage-gated potassium channel subunit Kv12.3) (FITC)

Cat. Num. AAA6312437

• Gene Names: KCNH4; BEC2; ELK1; Kv12.3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KCNH4; Polyclonal Antibody; NT (KCNH4; Potassium voltage-gated channel subfamily H member 4; Brain-specific eag-like channel 2; Ether-a-go-go-like potassium channel 1; Voltage-gated potassium channel subunit Kv12.3) (FITC); Voltage-gated potassium channel subunit Kv12.3; anti-KCNH4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-KCNH4 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KCNH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-100 amino acids from the N-terminal region of human KCNH4.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KCNH4 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the KCNH4 antibody detected the KCNH4 protein (arrow).)

Flow Cytometry (FC/FACS)

(KCNH4 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KCNH4 antibody

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit. The gene is brain-specific, and located in the neocortex and the striatum. It may be involved in cellular excitability of restricted neurons in the central nervous system.

Product Categories/Family for anti-KCNH4 antibody

Antibodies; Ion Channel

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Gutman, G.A., et al. Pharmacol. Rev. 57(4):473-508(2005). Zou, A., et al. Am. J. Physiol., Cell Physiol. 285 (6), C1356-C1366 (2003): Miyake, A., et al. J. Biol. Chem. 274(35):25018-25025(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 6912446
• NCBI GeneID: 23415
• NCBI Accession #: NP_036417.1
• NCBI GenBank Nucleotide #: NM_012285.2
• UniProt Accession #: Q9UQ05
• Molecular Weight: 111,693 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily H member 4
• NCBI Official Synonym Full Names: potassium voltage-gated channel subfamily H member 4
• NCBI Official Symbol: KCNH4
• NCBI Official Synonym Symbols: BEC2; ELK1; Kv12.3
• NCBI Protein Information: potassium voltage-gated channel subfamily H member 4
• UniProt Protein Name: Potassium voltage-gated channel subfamily H member 4
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: KCNH4
• UniProt Synonym Gene Names: BEC2; ELK channel 1; ELK1

NCBI Description

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit. The gene is brain-specific, and located in the neocortex and the striatum. It may be involved in cellular excitability of restricted neurons in the central nervous system. [provided by RefSeq, Jul 2008]

Uniprot Description

Kv12.3: Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits an outward current, but shows no inactivation. Channel properties may be modulated by cAMP and subunit assembly. Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv12.3/KCNH4 sub-subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: intracellular; plasma membrane; voltage-gated potassium channel complex

Molecular Function: phosphorelay sensor kinase activity; voltage-gated potassium channel activity

Biological Process: potassium ion transport; regulation of membrane potential; two-component signal transduction system (phosphorelay)