Rabbit anti-Human Karyopherin beta 1 Polyclonal Antibody | anti-KPNB1 antibody

Karyopherin beta 1, NT (Karyopherin Subunit beta-1, KPNB1, Importin beta 1, Importin Subunit beta-1, IMB1, IPO1, IPOB, Impnb, Importin 90, MGC2155, MGC2156, MGC2157, Nuclear Factor p97, NTF97, Pore Targeting Complex 97kD Subunit, PTAC97) (APC)

Cat. Num. AAA6484075

• Gene Names: KPNB1; IMB1; IPO1; IPOB; Impnb; NTF97
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Karyopherin beta 1; Polyclonal Antibody; NT (Karyopherin Subunit beta-1; KPNB1; Importin beta 1; Importin Subunit beta-1; IMB1; IPO1; IPOB; Impnb; Importin 90; MGC2155; MGC2156; MGC2157; Nuclear Factor p97; NTF97; Pore Targeting Complex 97kD Subunit; PTAC97) (APC); anti-KPNB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KPNB1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-KPNB1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human KPNB1 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KPNB1 Antibody (N-term) in Y79 cell line lysates (35ug/lane). KPNB1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with KPNB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(KPNB1 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-KPNB1 antibody

Antibodies; Transport Proteins

References

1. Nordgard, S.H., et.al., Genes Chromosomes Cancer 47(8), 680-696 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 19923142
• NCBI GeneID: 3837
• NCBI Accession #: NP_002256
• NCBI GenBank Nucleotide #: NM_002265.5
• UniProt Accession #: Q14974; Q14637; Q53XN2; Q96J27; B7ZAV6; D3DTT3
• Molecular Weight: 99.6 kDa (899aa)
• NCBI Official Full Name: importin subunit beta-1 isoform 1
• NCBI Official Synonym Full Names: karyopherin subunit beta 1
• NCBI Official Symbol: KPNB1
• NCBI Official Synonym Symbols: IMB1; IPO1; IPOB; Impnb; NTF97
• NCBI Protein Information: importin subunit beta-1
• UniProt Protein Name: Importin subunit beta-1
• Protein Family: Importin
• UniProt Gene Name: KPNB1
• UniProt Synonym Gene Names: NTF97; PTAC97
• UniProt Entry Name: IMB1_HUMAN

NCBI Description

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]

Uniprot Description

KPNB1: a nuclear import protein nuclear protein import that functions either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re- exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus. Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. Two isoforms of the human protein have been reported. Belongs to the importin beta family.

Protein type: Karyopherin; Nuclear import

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: nucleoplasm; nuclear membrane; membrane; cytoplasm; nuclear pore; nuclear envelope; cytosol

Molecular Function: protein domain specific binding; protein binding; enzyme binding; zinc ion binding; nuclear localization sequence binding; Ran GTPase binding; protein transporter activity

Biological Process: ribosomal protein import into nucleus; viral reproduction; apoptosis; protein import into nucleus; protein import into nucleus, translocation; cytokine and chemokine mediated signaling pathway; viral infectious cycle; NLS-bearing substrate import into nucleus; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis