Western Blot (WB)
((1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat KAP1/TRIM28 Polyclonal Antibody | anti-TRIM28 antibody
Goat anti-KAP1/TRIM28 Antibody
Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration, 2-4ug/ml. This product has been successfully used on Human gastric cancer surgical specimens: Yokoe et al, Ann Surg Oncol.2010 Mar;17(3): 821-8. (PMID: 19898899). Recommended concentration: 5ug/ml.
Western Blot: Approx 120kDa band observed in lysates of cell line HepG2 (calculated MW of 88.5kDa according to NP_005753.1). The higher observed MW is explained by sumoylated protein. Recommended concentration: 1-3ug/ml.
Immunofluorescence: Successfully used on mouse embryo at the late zygote stage, 5ug/ml (personal communication).
Chromatin immunoprecipitation: This antibody has been successfully used in ChIP for K562 cells as described in the following paper: Frietze et al, PLoS One.2010 Dec 8;5(12): e15082.; PMID: 21170338.
Western Blot (WB)
((1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 9, HRP-staining.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 9, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
Uniprot Description
TIF1B: an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
Protein type: Ligase; Transcription, coactivator/corepressor; Ubiquitin ligase; Protein kinase, atypical; Nuclear receptor co-regulator; Ubiquitin conjugating system; Kinase, protein; EC 6.3.2.-; ATYPICAL group; TIF1 family
Chromosomal Location of Human Ortholog: 19q13.4
Cellular Component: nuclear heterochromatin; nucleoplasm; nucleus
Molecular Function: DNA binding; ligase activity; protein binding; protein kinase activity; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding
Biological Process: chromatin modification; convergent extension involved in axis elongation; DNA methylation during embryonic development; DNA repair; embryo implantation; epithelial to mesenchymal transition; innate immune response; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of DNA repair; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; protein oligomerization; protein sumoylation; protein ubiquitination; Ras protein signal transduction; transcription initiation from RNA polymerase II promoter
Research Articles on TRIM28
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Product Notes
The TRIM28 trim28 (Catalog #AAA420240) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-KAP1/TRIM28 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KAP1/TRIM28 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP). Peptide ELISA: Antibody detection limit dilution 1: 4000. Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration, 2-4ug/ml. This product has been successfully used on Human gastric cancer surgical specimens: Yokoe et al, Ann Surg Oncol.2010 Mar;17(3): 821-8. (PMID: 19898899). Recommended concentration: 5ug/ml. Western Blot: Approx 120kDa band observed in lysates of cell line HepG2 (calculated MW of 88.5kDa according to NP_005753.1). The higher observed MW is explained by sumoylated protein. Recommended concentration: 1-3ug/ml. Immunofluorescence: Successfully used on mouse embryo at the late zygote stage, 5ug/ml (personal communication). Chromatin immunoprecipitation: This antibody has been successfully used in ChIP for K562 cells as described in the following paper: Frietze et al, PLoS One.2010 Dec 8;5(12): e15082.; PMID: 21170338. Researchers should empirically determine the suitability of the TRIM28 trim28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SSQELSGGPG DGP. It is sometimes possible for the material contained within the vial of "KAP1/TRIM28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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