Western Blot (WB)
(Anti-Kallikrein 1 antibody, MBS175345, Western blottingLane 1: Recombinant Mouse KLK1 Protein 10ngLane 2: Recombinant Mouse KLK1 Protein 5ngLane 3: Recombinant Mouse KLK1 Protein 2.5ngLane 4: Recombinant Mouse KLK1 Protein 1.25ng )
Rabbit anti-Mouse Kallikrein 1 Polyclonal Antibody | anti-KLK1 antibody
Anti-Kallikrein 1 antibody
Gene Names
Klk1; Kal; Klk6; mGk-6; Klk1b6; 0610007D04Rik
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
Kallikrein 1; Polyclonal Antibody; Anti-Kallikrein 1 antibody; Kallikrein-1; kallikrein 1; Glandular kallikrein 1 antibody; hK 1 antibody; hK1 antibody; Kallikrein serine protease 1 antibody; Kallikrein-1 antibody; Kidney/pancreas/salivary gland kallikrein antibody; Klk 6 antibody; KLK1 antibody; KLK1_HUMAN antibody; Klk6 antibody; KLKR antibody; Tissue kallikrein antibody; anti-KLK1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
261
Applicable Applications for anti-KLK1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB:
Concentration:0.1-0.5ug/ml
Tested Species: Ms
IHC-P:
Concentration: 0.5-1ug/ml
Tested Species: MS
Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Concentration:0.1-0.5ug/ml
Tested Species: Ms
IHC-P:
Concentration: 0.5-1ug/ml
Tested Species: MS
Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Kallikrein 1 (243-261aa YTRVLNFNTWIRETMAEND).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-Kallikrein 1 antibody, MBS175345, Western blottingLane 1: Recombinant Mouse KLK1 Protein 10ngLane 2: Recombinant Mouse KLK1 Protein 5ngLane 3: Recombinant Mouse KLK1 Protein 2.5ngLane 4: Recombinant Mouse KLK1 Protein 1.25ng )
Western Blot (WB)
(Anti-Kallikrein 1 antibody, MBS175345, Western blottingLane 1: Recombinant Mouse KLK1 Protein 10ngLane 2: Recombinant Mouse KLK1 Protein 5ngLane 3: Recombinant Mouse KLK1 Protein 2.5ngLane 4: Recombinant Mouse KLK1 Protein 1.25ng )
Immunohistochemistry (IHC)
(Anti-Kallikrein 1 antibody, MBS175345, IHC(P)IHC(P): Mouse Kidney Tissue )
Immunohistochemistry (IHC)
(Anti-Kallikrein 1 antibody, MBS175345, IHC(P)IHC(P): Mouse Kidney Tissue )
Related Product Information for anti-KLK1 antibody
Description: Rabbit IgG polyclonal antibody for Kallikrein-1(KLK1) detection. Tested with WB, IHC-P in Mouse.
Background: KLK1(KALLIKREIN 1), also called KLKR, is a protein that in humans is encoded by the KLK1 gene. KLK1 is a member of the peptidase S1 family. KLK1 is a serine protease that generates Lys-bradykinin by specific proteolysis of kininogen-1. The KLK1 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19 and its exact cytogenetic location is 19q13.33. The KLK1 gene contains 5 coding exons. And KLK1 is the most centromeric gene in the cluster. Mice lacking tissue kallikrein are unable to generate significant levels of kinins in most tissues and develop cardiovascular abnormalities early in adulthood despite normal blood pressure. The protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen.
Background: KLK1(KALLIKREIN 1), also called KLKR, is a protein that in humans is encoded by the KLK1 gene. KLK1 is a member of the peptidase S1 family. KLK1 is a serine protease that generates Lys-bradykinin by specific proteolysis of kininogen-1. The KLK1 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19 and its exact cytogenetic location is 19q13.33. The KLK1 gene contains 5 coding exons. And KLK1 is the most centromeric gene in the cluster. Mice lacking tissue kallikrein are unable to generate significant levels of kinins in most tissues and develop cardiovascular abnormalities early in adulthood despite normal blood pressure. The protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
28,775 Da
NCBI Official Full Name
Kallikrein-1
NCBI Official Synonym Full Names
kallikrein 1
NCBI Official Symbol
Klk1
NCBI Official Synonym Symbols
Kal; Klk6; mGk-6; Klk1b6; 0610007D04Rik
NCBI Protein Information
kallikrein-1; KAL-B; kallikrein 6; renal kallikrein; tissue kallikrein-6; true tissue kallikrein; glandular kallikrein K1; kallikrein renal/pancreas/salivary
UniProt Protein Name
Kallikrein-1
Protein Family
UniProt Gene Name
Klk1
UniProt Synonym Gene Names
Klk-6; Klk6; mGK-6
UniProt Entry Name
KLK1_MOUSE
Uniprot Description
KLK1: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Belongs to the peptidase S1 family. Kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.21.35; Protease
Cellular Component: nucleus
Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity
Biological Process: proteolysis
Research Articles on KLK1
Similar Products
Product Notes
The KLK1 klk1 (Catalog #AAA175345) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Kallikrein 1 antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Kallikrein 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: Concentration:0.1-0.5ug/ml Tested Species: Ms IHC-P: Concentration: 0.5-1ug/ml Tested Species: MS Antigen Retrieval: By Heat Tested Species: In-house tested species with positive results. Predicted Species: Species to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the KLK1 klk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kallikrein 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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