Chemiluminescence
((2ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Kalirin Polyclonal Antibody | anti-Kalrn antibody
Kalirin (Huntington-associated Protein-interacting Protein, Protein Duo, Serine/Threonine-protein Kinase with Dbl- and Pleckstrin Homology Domain, DUET, DUO, HAPIP, TRAD)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: ELISA: 1:8000
Western Blot: 0.5-1.5ug/ml
Chemiluminescence
((2ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Chemiluminescence
((2ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
binds the human huntingtin associated protein Hap1 in vitro; has GDP/GTP exchange factor activity for Rho subfamily GTP-binding proteins [RGD, Feb 2006]
Uniprot Description
Function: Promotes the exchange of GDP by GTP. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity. Isoforms 1 and 7 are necessary for neuronal development and axonal outgrowth. Isoform 6 is required for dendritic spine formation. Ref.3 Ref.5 Ref.7
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Cofactor: Magnesium
By similarity.
Subunit structure: Interacts with the C-terminal of peptidylglycine alpha-amidating monooxygenase (PAM) and with the huntingtin-associated protein 1 (HAP1). Interacts with FASLG
By similarity. Ref.2 Ref.8
Subcellular location: Cytoplasm. Cytoplasm › cytoskeleton. Note: Isoform 6 is largely associated with synaptosomal membranes and to punctate structures in cortical neurons. Isoforms 1 and 7 are expressed in neuronal cell bodies, isoform 7 is also found in neuronal processes. Ref.3 Ref.4
Tissue specificity: Highly expressed in the brain and nervous system. Isoform 6 is highly enriched in the postsynaptic density fraction of the cerebral cortex. Ref.3 Ref.4
Developmental stage: Isoforms 1 and 7 are prevelant 2 dpp, isoform 6 is not detectable until 2 weeks after birth. Ref.7
Domain: The two GEF domains catalyze nucleotide exchange for RAC1 and RhoA which are bound by DH1 and DH2 respectively. The two GEF domains appear to play differing roles in neuronal development and axonal outgrowth. SH3 1 binds to the first GEF domain inhibiting GEF activity only when in the presence of a PXXP peptide, suggesting that the SH3 domain/peptide interaction mediates binding to GEF1. CRK1 SH3 domain binds to and inhibits GEF1 activity. Ref.7 Ref.8
Post-translational modification: Autophosphorylated
By similarity.
Miscellaneous: Called DUO because the encoded protein is closely related to but shorter than TRIO.
Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.Contains 1 CRAL-TRIO domain.Contains 2 DH (DBL-homology) domains.Contains 1 fibronectin type-III domain.Contains 1 Ig-like C2-type (immunoglobulin-like) domain.Contains 2 PH domains.Contains 1 protein kinase domain.Contains 2 SH3 domains.Contains 5 spectrin repeats.
Research Articles on Kalrn
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Product Notes
The Kalrn kalrn (Catalog #AAA646423) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Kalirin (Huntington-associated Protein-interacting Protein, Protein Duo, Serine/Threonine-protein Kinase with Dbl- and Pleckstrin Homology Domain, DUET, DUO, HAPIP, TRAD) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kalirin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:8000 Western Blot: 0.5-1.5ug/ml. Researchers should empirically determine the suitability of the Kalrn kalrn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kalirin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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