Rabbit anti-Human, Mouse K1199 Polyclonal Antibody | anti-K1199 antibody

K1199, CT (KIAA1199, Protein KIAA1199) (PE)

Cat. Num. AAA6311938

• Gene Names: CEMIP; CCSP1; TMEM2L; KIAA1199
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: K1199; Polyclonal Antibody; CT (KIAA1199; Protein KIAA1199) (PE); KIAA1199; Protein KIAA1199; anti-K1199 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-K1199 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: K1199 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1292-1321 amino acids from the C-terminal region of human K1199.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(K1199 Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the K1199 antibody detected the K1199 protein (arrow).)

Product Categories/Family for anti-K1199 antibody

Antibodies; KIAA; orf

References

Rose, J. Phd, et al. Mol. Med. (2010). In press: Matsuzaki, S., et al. Ann. Surg. Oncol. 16(7):2042-2051(2009). Michishita, E., et al. Cancer Lett. 239(1):71-77(2006). Guo, J., et al. FEBS Lett. 580(2):581-584(2006). Abe, S., et al. Am. J. Hum. Genet. 72(1):73-82(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 38638698
• NCBI GeneID: 57214
• NCBI Accession #: NP_061159.1
• NCBI GenBank Nucleotide #: NM_018689.2
• UniProt Accession #: Q8WUJ3; Q6L9J5; Q9H1K5; Q9NPN9; Q9ULM1
• Molecular Weight: 152,998 Da
• NCBI Official Full Name: cell migration-inducing and hyaluronan-binding protein
• NCBI Official Synonym Full Names: cell migration inducing protein, hyaluronan binding
• NCBI Official Symbol: CEMIP
• NCBI Official Synonym Symbols: CCSP1; TMEM2L; KIAA1199
• NCBI Protein Information: cell migration-inducing and hyaluronan-binding protein; colon cancer secreted protein 1
• UniProt Protein Name: Cell migration-inducing and hyaluronan-binding protein
• UniProt Gene Name: CEMIP
• UniProt Synonym Gene Names: KIAA1199

Uniprot Description

Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing.