Rabbit JunB Polyclonal Antibody | anti-JunB antibody

JunB (D253) Polyclonal Antibody

Cat. Num. AAA3000640

• Gene Names: JUNB; AP-1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: JunB; Polyclonal Antibody; JunB (D253) Polyclonal Antibody; Jun B; Jun B proto oncogene; Transcription factor jun-B; anti-JunB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: JunB (D253) polyclonal antibody detects endogenous levels of JunB protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 347

• Applicable Applications for anti-JunB antibody: Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: IHC: 1:50-1:200; IP: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 220-270 of Human JunB.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of JunB (D253) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-JunB antibody

The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein along with c-Fos is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in ranscriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.

NCBI and Uniprot Product Information

• NCBI GI #: 4504809
• NCBI GeneID: 3726
• NCBI Accession #: NP_002220.1
• NCBI GenBank Nucleotide #: NP_002220.1
• UniProt Accession #: P17275
• Molecular Weight: ~ 43kDa
• NCBI Official Full Name: transcription factor jun-B
• NCBI Official Synonym Full Names: JunB proto-oncogene, AP-1 transcription factor subunit
• NCBI Official Symbol: JUNB
• NCBI Official Synonym Symbols: AP-1
• NCBI Protein Information: transcription factor jun-B
• UniProt Protein Name: Transcription factor jun-B
• Protein Family: Transcription factor
• UniProt Gene Name: JUNB
• UniProt Entry Name: JUNB_HUMAN

Uniprot Description

JunB: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Binds DNA as an homodimer or as an heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). By growth factors. Belongs to the bZIP family. Jun subfamily.

Protein type: Transcription factor; Motility/polarity/chemotaxis; Oncoprotein

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; chromatin

Molecular Function: protein binding; DNA binding; transcription coactivator activity; double-stranded DNA binding; transcription factor activity; transcription corepressor activity

Biological Process: response to drug; transcription initiation from RNA polymerase II promoter; response to peptide hormone stimulus; response to light stimulus; response to cAMP; transcription, DNA-dependent; regulation of cell cycle; embryonic process involved in female pregnancy; decidualization; osteoclast differentiation; cellular response to hormone stimulus; trophectodermal cell differentiation; regulation of transcription from RNA polymerase II promoter; osteoblast differentiation; osteoblast proliferation; response to mechanical stimulus; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; vasculogenesis; response to progesterone stimulus; response to corticosterone stimulus