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Western Blot (WB) (Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10% SDS PAGE gel and JNK1 antibody at a dilution of 1:1000)

Rabbit JNK1 Polyclonal Antibody | anti-JNK1 antibody

JNK1 Antibody

Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
JNK1; Polyclonal Antibody; JNK1 Antibody; Affinity Purified Rabbit Polyclonal JNK1 Antibody; Polyclonal JNK1 antibody; Anti-JNK1 antibody; JNK21B1/2 antibody; SAPK1 antibody; c-Jun N-terminal kinase 1 antibody; JUN N-terminal kinase antibody; JNK-1 antibody; mitogen-activated protein kinase 8 isoform JNK1 alpha1 antibody; MAP kinase 8 antibody; PRKM8 antibody; JNK 1; JNK antibody; JNK-1; mitogen-activated protein kinase 8 isoform JNK1 beta2 antibody; JNK1 antibody; stress-activated protein kinase 1c antibody; stress-activated protein kinase 1 antibody; JNK1A2 antibody; JNK 1 antibody; MAPK8 antibody; mitogen-activated protein kinase 8 antibody; anti-JNK1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.4 mg/ml (varies by lot)
Sequence Length
384
Applicable Applications for anti-JNK1 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
IF: 1:100-1:1000
ICC: 1:100-1:1000
WB: 1:500-1:3000
Immunogen
JNK1 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 5 and 300 of JNK1
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10% SDS PAGE gel and JNK1 antibody at a dilution of 1:1000)

Western Blot (WB) (Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10% SDS PAGE gel and JNK1 antibody at a dilution of 1:1000)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: Raji) using a 10% SDS PAGE gel and JNK1 antibody at a dilution of 1:1000)

Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: Raji) using a 10% SDS PAGE gel and JNK1 antibody at a dilution of 1:1000)

Immunofluorescence (IF)

(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using JNK1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)

Immunofluorescence (IF) (Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using JNK1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Related Product Information for anti-JNK1 antibody
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.
Product Categories/Family for anti-JNK1 antibody

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
48 kDa
NCBI Official Full Name
JNK1

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Product Notes

The JNK1 (Catalog #AAA5311792) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's JNK1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB). IF: 1:100-1:1000 ICC: 1:100-1:1000 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the JNK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JNK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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