Rabbit JMJD5 Polyclonal Antibody | anti-JMJD5 antibody

JMJD5 Antibody

Cat. Num. AAA150216

• Gene Names: KDM8; JMJD5
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: JMJD5 Antibody is affinity chromatography purified via peptide column.
• Synonyms: JMJD5; Polyclonal Antibody; JMJD5 Antibody; Lysine-specific demethylase 8; JmjC domain-containing protein 5; jumonji domain containing 5; anti-JMJD5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: JMJD5 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 454

• Applicable Applications for anti-JMJD5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: JMJD5 antibody can be used for detection of JMJD5 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: JMJD5 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human JMJD5.
• Buffer: JMJD5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: JMJD5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of JMJD5 in human liver tissue lysate with JMJD5 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of JMJD5 in rat liver tissue with JMJD5 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of JMJD5 in Rat Liver cells with JMJD5 antibody at 20 μg/mL.)

Related Product Information for anti-JMJD5 antibody

JMJD5 Antibody: The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. While several JMJD proteins have been identified as being involved in chromatin regulation, histone demethylation and development, the function of JMJD5 has not been identified. JMJD5 is expressed in multiple tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 223942018
• NCBI GeneID: 79831
• NCBI Accession #: NP_001138820
• NCBI GenBank Nucleotide #: NM_001145348.1
• UniProt Accession #: Q8N371; Q6VAK5; Q9H8B1; B4DLU9
• Molecular Weight: 50,852 Da
• NCBI Official Full Name: lysine-specific demethylase 8 isoform 1
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 8
• NCBI Official Symbol: KDM8
• NCBI Official Synonym Symbols: JMJD5
• NCBI Protein Information: lysine-specific demethylase 8; jumonji domain containing 5; jmjC domain-containing protein 5; jumonji domain-containing protein 5
• UniProt Protein Name: Lysine-specific demethylase 8
• UniProt Gene Name: KDM8
• UniProt Synonym Gene Names: JMJD5
• UniProt Entry Name: KDM8_HUMAN

NCBI Description

This gene likely encodes a histone lysine demethylase. Studies of a similar protein in mouse indicate a potential role for this protein as a tumor suppressor. Alternatively spliced transcript variants have been described.[provided by RefSeq, Feb 2009]

Uniprot Description

JMJD5: Histone demethylase required for G2/M phase cell cycle progression. Specifically demethylates dimethylated 'Lys-36' (H3K36me2) of histone H3, an epigenetic repressive mark, thereby acting as a transcription activator. Regulates expression of CCNA1 (cyclin-A1), leading to regulate cancer cell proliferation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Demethylase; EC 1.14.11.27; Cell cycle regulation

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: nucleus

Molecular Function: histone demethylase activity (H3-K36 specific); metal ion binding; chromatin binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; G2/M transition of mitotic cell cycle