Rabbit JDP2 Polyclonal Antibody | anti-JDP2 antibody

Phospho-JDP2 (Thr148) Antibody

Cat. Num. AAA9614277

• Gene Names: JDP2; JUNDM2
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (89%)
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: JDP2; Polyclonal Antibody; Phospho-JDP2 (Thr148) Antibody; Jdp2; JDP2_HUMAN; Jun dimerization protein 2; JUNDM2; Progesterone receptor co-activator; anti-JDP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (89%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-JDP2 (Thr148) Antibody detects endogenous levels of JDP2 only when phosphorylated at Thr148.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-JDP2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IF/ICC: 1:100-1:500; IHC: 1:50-1:200; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human JDP2 around the phosphorylation site of Thr148.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylation of Thr-148 by MAPK8 in response to different stress conditions such as, UV irradiation, oxidatives stress and anisomycin treatments.Polyubiquitinated; probably by IRF2BP1.
• Subunit Structure: Forms a homodimer or heterodimer with JUN, JUNB, JUND, CEBPG and ATF2 thereby inhibiting transactivation by JUN, ATF2 and CEBPG (By similarity). Binds multiple DNA elements such as cAMP-response element (CRE) and TPA response element (TRE) either as homodimer or heterodimer (By similarity). Interacts with IRF2BP1.
• Similarity: Belongs to the bZIP family. ATF subfamily.
• Subcellular Location: Nucleus.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from C6 cells LPS, using Phospho-JDP-2 (Thr148) Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of JDP-2 (Phospho-Thr148) using UV treated A549 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Immunohistochemistry (IHC)

(At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Testing Data

(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Related Product Information for anti-JDP2 antibody

Component of the AP-1 transcription factor that represses transactivation mediated by the Jun family of proteins. Involved in a variety of transcriptional responses associated with AP-1 such as UV-induced apoptosis, cell differentiation, tumorigenesis and antitumogeneris. Can also function as a repressor by recruiting histone deacetylase 3/HDAC3 to the promoter region of JUN. May control transcription via direct regulation of the modification of histones and the assembly of chromatin.

NCBI and Uniprot Product Information

• NCBI GI #: 205277417
• NCBI GeneID: 122953
• NCBI Accession #: NP_001128519.1
• NCBI GenBank Nucleotide #: NM_001135047.1
• UniProt Accession #: Q8WYK2; O95430; Q9UIE4
• Molecular Weight: 18,704 Da
• NCBI Official Full Name: jun dimerization protein 2 isoform a
• NCBI Official Synonym Full Names: Jun dimerization protein 2
• NCBI Official Symbol: JDP2
• NCBI Official Synonym Symbols: JUNDM2
• NCBI Protein Information: jun dimerization protein 2; progesterone receptor co-activator
• UniProt Protein Name: Jun dimerization protein 2
• Protein Family: Jun dimerization protein
• UniProt Gene Name: JDP2
• UniProt Entry Name: JDP2_HUMAN

Uniprot Description

JDP2: a transcriptional coregulator that may play a role in progesterone receptor signaling. a JDP-2 lacks an intrinsic activation domain and interacts directly with other coactivators including CBP and pCAF.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; sequence-specific DNA binding; protein heterodimerization activity; double-stranded DNA binding; chromatin binding; transcription factor activity

Biological Process: positive regulation of histone deacetylation; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation