Rabbit anti-Human JAML1 Polyclonal Antibody | anti-JAML1 antibody

JAML1 Antibody

Cat. Num. AAA9604065

• Gene Names: JAML; AMICA; Gm638; AMICA1; CREA7-1; CREA7-4
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: JAML1; Polyclonal Antibody; JAML1 Antibody; adhesion molecule AMICA; Adhesion molecule interacting with CXADR antigen 1; adhesion molecule interacts with CXADR antigen 1; adhesion molecule that interacts with CXADR antigen 1; AMICA; Amica1; CREA7-1; CREA7-4; Dendritic cell-specific protein CREA7-1; dendritic-cell specific protein CREA7-1; dendritic-cell specific protein CREA7-4; FLJ37080; Gm638; JAML1_HUMAN; junctional adhesion molecule; Junctional adhesion molecule-like; Junctional adhesion molecule-like precursor; MGC118814; MGC118815; UNQ722/PRO1387; anti-JAML1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: JAML1 antibody detects endogenous levels of total JAML1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 394

• Applicable Applications for anti-JAML1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide
• Subcellular Location: Plasma Membrane
• Tissue Specificity: Expression is restricted to the hematopoietic tissues with the exception of liver. Expressed in fetal liver, spleen and thymus. Preferentially expressed by mature leukocytes (at protein level).
• Conjugation: Unconjugated

Western Blot (WB)

(Western blot analysis of JAML1 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(MBS9604065 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-JAML1 antibody

Function: Transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. It also controls the transmigration of leukocytes within epithelial and endothelial tissues through adhesive interactions with epithelial and endothelial CXADR.
Subunit Structure: Homodimer; active form in leukocyte-endothelial cell adhesion. Interacts (homodimeric form) with CXADR. Interacts (via cytoplasmic domain) with the PI3 kinase; upon CXADR-binding. Interacts with ITGA4 and ITGB1; integrin alpha-4/beta-1 may regulate leukocyte to endothelial cells adhesion by controlling JAML homodimerization.
Similarity: The Ig-like V-type domain 1 mediates interaction with CXADR (By similarity). The Ig-like V-type domain 2 may also play a role in the interaction (PubMed:15800062). Belongs to the immunoglobulin superfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 148664207
• NCBI GeneID: 120425
• NCBI Accession #: NP_001091996.1
• NCBI GenBank Nucleotide #: NM_001098526.1
• UniProt Accession #: Q86YT9; Q496M1; Q5DTC6; Q7Z499; Q8N9I7; Q8NF70; B0YIV1; B0YIV2
• Molecular Weight: Predicted: 45 kDa
• NCBI Official Full Name: junctional adhesion molecule-like isoform 1
• NCBI Official Synonym Full Names: junction adhesion molecule like
• NCBI Official Symbol: JAML
• NCBI Official Synonym Symbols: AMICA; Gm638; AMICA1; CREA7-1; CREA7-4
• NCBI Protein Information: junctional adhesion molecule-like
• UniProt Protein Name: Junctional adhesion molecule-like
• UniProt Gene Name: JAML
• UniProt Synonym Gene Names: AMICA1

Uniprot Description

Transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. It also controls the transmigration of leukocytes within epithelial and endothelial tissues through adhesive interactions with epithelial and endothelial CXADR.