Western Blot (WB)
(Figure 1. Western blot analysis of JAM-A/F11R using anti-JAM-A/F11R antibody (MBS1753488).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human CACO-2 whole cell lysatesLane 3: human MDA-MB-453 whole cell lysatesLane 4: human HEPG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-JAM-A/F11R antigen affinity purified polyclonal antibody (Catalog # MBS1753488) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for JAM-A/F11R at approximately 41KD. The expected band size for JAM-A/F11R is at 41KD. )
Rabbit anti-Human JAM-A/F11R Polyclonal Antibody | anti-F11R antibody
Anti-JAM-A/F11R Antibody
ICC/IF: 5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Western Blot (WB)
(Figure 1. Western blot analysis of JAM-A/F11R using anti-JAM-A/F11R antibody (MBS1753488).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human CACO-2 whole cell lysatesLane 3: human MDA-MB-453 whole cell lysatesLane 4: human HEPG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-JAM-A/F11R antigen affinity purified polyclonal antibody (Catalog # MBS1753488) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for JAM-A/F11R at approximately 41KD. The expected band size for JAM-A/F11R is at 41KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of JAM-A/F11R using anti-JAM-A/F11R antibody (MBS1753488).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human CACO-2 whole cell lysatesLane 3: human MDA-MB-453 whole cell lysatesLane 4: human HEPG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-JAM-A/F11R antigen affinity purified polyclonal antibody (Catalog # MBS1753488) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for JAM-A/F11R at approximately 41KD. The expected band size for JAM-A/F11R is at 41KD. )
Immunofluorescence (IF)
(Figure 2. IF analysis of JAM-A/F11R using anti- JAM-A/F11R antibody (MBS1753488).JAM-A/F11R was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- JAM-A/F11R Antibody (MBS1753488) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Immunofluorescence (IF)
(Figure 2. IF analysis of JAM-A/F11R using anti- JAM-A/F11R antibody (MBS1753488).JAM-A/F11R was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- JAM-A/F11R Antibody (MBS1753488) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of HEPG2 cells using anti-JAM-A/F11R antibody (MBS1753488).Overlay histogram showing HEPG2 cells stained with MBS1753488 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JAM-A/F11R Antibody (MBS1753488, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of HEPG2 cells using anti-JAM-A/F11R antibody (MBS1753488).Overlay histogram showing HEPG2 cells stained with MBS1753488 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JAM-A/F11R Antibody (MBS1753488, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Arrate, M. P., Rodriguez, J. M., Tran, T. T., Brock, T. A., Cunningham, S. A. Cloning of human junctional adhesion molecule 3 (JAM3) and its identification as the JAM2 counter-receptor. J. Biol. Chem. 276: 45826-45832, 2001.
3. Barton, E. S., Forrest, J. C., Connolly, J. L., Chappell, J. D., Liu, Y., Schnell, F. J., Nusrat, A., Parkos, C. A., Dermody, T. S. Junction adhesion molecule is a receptor for reovirus. Cell 104: 441-451, 2001.
NCBI and Uniprot Product Information
NCBI Description
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008]
Uniprot Description
JAM-A: Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. Belongs to the immunoglobulin superfamily.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 1q21.2-q21.3
Cellular Component: microtubule cytoskeleton; tight junction; integral to membrane; plasma membrane; intercellular junction; cytoplasmic vesicle; cell junction
Molecular Function: protein binding; PDZ domain binding
Biological Process: intercellular junction assembly and maintenance; response to radiation; extracellular matrix organization and biogenesis; viral reproduction; transforming growth factor beta receptor signaling pathway; actomyosin structure organization and biogenesis; inflammatory response; cell adhesion; blood coagulation; leukocyte migration; positive regulation of blood pressure; intestinal absorption
Research Articles on F11R
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Product Notes
The F11R f11r (Catalog #AAA1753488) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-JAM-A/F11R Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JAM-A/F11R can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM). WB: 0.25-0.5ug/ml|Human| ICC/IF: 5ug/ml|Human| FC/FACS/FCM: 1-3ug/1x106 cells|Human|. Researchers should empirically determine the suitability of the F11R f11r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JAM-A/F11R, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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