Western Blot (WB)
(Western blot analysis of JAGN1 in MCF7 cell lysate with JAGN1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Rabbit JAGN1 Polyclonal Antibody | anti-JAGN1 antibody
JAGN1 Antibody
Gene Names
JAGN1; SCN6; GL009
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity chromatography purified via peptide column.
Synonyms
JAGN1; Polyclonal Antibody; JAGN1 Antibody; Jagunal homolog 1; anti-JAGN1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
JAGN1 antibody is human, mouse and rat reactive. At least four isoforms of JAGN1 are known to exist; this antibody will detect all but isoform 2.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
183
Applicable Applications for anti-JAGN1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
JAGN1 antibody can be used for detection of JAGN1 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL.
Immunogen
JAGN1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human JAGN1.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
Mouse Colon Tissue Lysate
Conjugate
Unconjugated
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of JAGN1 in MCF7 cell lysate with JAGN1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of JAGN1 in MCF7 cell lysate with JAGN1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry of JAGN1 in rat colon tissue with JAGN1 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of JAGN1 in rat colon tissue with JAGN1 antibody at 2.5 μg/mL.)
Related Product Information for anti-JAGN1 antibody
JAGN1 is the human ortholog of a gene originally identified in Drosophila melanogaster. It is a transmembrane protein that functions in the early secretory pathway and is necessary for neutrophil differentiation and survival. Individuals with mutations in the JAGN1 gene causes aberrant myeloid cell homeostasis and severe congenital neutropenia (SCN) (1). JAGN1-mutant granulocytes are characterized by ultrastructural defects, aberrant N-glycosylation of multiple proteins, and increased apoptosis (1). Mice lacking this gene could not mount and efficient neutrophil-dependent immune response to a human fungal pathogen (2).
Product Categories/Family for anti-JAGN1 antibody
References
Boztug K, Jarvinen PM, Salzer E, et al. JAGN1 deficiency causes aberrant myeloid cell homeostasis and congenital neutropenia. Nat. Genet. 2014; 46:1021-7. Wirnsberger G, Zwolanek F, Stadlmann J, et al. Jagunal homolog 1 is a critical regulator of neutrophil function in fungal host defense. Nat. Genet. 2014; 46:1028-33.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 20 kDa
Observed: 25 kDa
Observed: 25 kDa
NCBI Official Full Name
protein jagunal homolog 1
NCBI Official Synonym Full Names
jagunal homolog 1
NCBI Official Symbol
JAGN1
NCBI Official Synonym Symbols
SCN6; GL009
NCBI Protein Information
protein jagunal homolog 1
UniProt Protein Name
Protein jagunal homolog 1
Protein Family
UniProt Gene Name
JAGN1
UniProt Entry Name
JAGN1_HUMAN
NCBI Description
The protein encoded by this gene is a transmembrane protein. It functions in the early secretory pathway and is necessary for neutrophil differentiation and survival. Mutations in this gene result in severe congenital neutropenia. [provided by RefSeq, Oct 2014]
Uniprot Description
JAGN1: May be required for endoplasmic reticulum organization. Belongs to the jagunal family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3p25.2
Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane
Molecular Function: protein binding
Biological Process: defense response to fungus; endoplasmic reticulum organization and biogenesis; exocytosis; neutrophil differentiation; neutrophil mediated immunity; protein transport; vesicle-mediated transport
Disease: Neutropenia, Severe Congenital, 6, Autosomal Recessive
Research Articles on JAGN1
Similar Products
Product Notes
The JAGN1 jagn1 (Catalog #AAA154358) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JAGN1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's JAGN1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). JAGN1 antibody can be used for detection of JAGN1 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. Researchers should empirically determine the suitability of the JAGN1 jagn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JAGN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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