Western Blot (WB)
(Western blot analysis of JAD1B expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse JAD1B Polyclonal Antibody | anti-JAD1B antibody
JAD1B Antibody
Gene Names
KDM5B; CT31; PLU1; PUT1; MRT65; PLU-1; JARID1B; PPP1R98; RBP2-H1; RBBP2H1A
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
JAD1B; Polyclonal Antibody; JAD1B Antibody; Cancer/testis antigen 31; CT31; Histone demethylase JARID1B; JARID1B; Jumonji AT rich interactive domain 1B; Jumonji/ARID domain-containing protein 1B; Kdm5b; KDM5B_HUMAN; Lysine (K) specific demethylase 5B; Lysine demethylase 5B; Lysine specific demethylase 5B; Lysine-specific demethylase 5B; PLU-1; PLU1; PPP1R98; Protein phosphatase 1 regulatory subunit 98; PUT1; putative DNA/chromatin binding motif; RBBP2H1A; RBP2 like; RBP2-H1; Retinoblastoma-binding protein 2 homolog 1; Retinoblastoma-binding protein 2 homolog 1A; anti-JAD1B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
JAD1B antibody detects endogenous levels of total JAD1B
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1580
Applicable Applications for anti-JAD1B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Tissue Specificity
Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (92%), Bovine (92%), Horse (100%), Sheep (92%), Rabbit (100%), Dog (100%), Chicken (83%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of JAD1B expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of JAD1B expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-JAD1B antibody
Function: Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed:24952722, PubMed:27214403, PubMed:28262558). Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5 (PubMed:24952722). In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-ARNTL/BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).
Subunit Structure: Interacts with FOXG1B, PAX9, MYC, MYCN and RB1. Interacts with HDAC1, HDAC4, HDAC5 and HDAC7. Interacts (via PHD-type 1 zinc finger) with histone H3 unmodified at 'Lys-4'; the interaction is inhibited when histone H3 is methylated at 'Arg-2' or 'Lys-4' (PubMed:24952722).
Similarity: Both the JmjC domain and the JmjN domain are required for enzymatic activity. However ARID and PHD-type 1 domain are not required for activity per se but contributed to recognition of the H3(1-21)K4me2 substrate peptide.The 2 first PHD-type zinc finger domains are required for transcription repression activity. Belongs to the JARID1 histone demethylase family.
Subunit Structure: Interacts with FOXG1B, PAX9, MYC, MYCN and RB1. Interacts with HDAC1, HDAC4, HDAC5 and HDAC7. Interacts (via PHD-type 1 zinc finger) with histone H3 unmodified at 'Lys-4'; the interaction is inhibited when histone H3 is methylated at 'Arg-2' or 'Lys-4' (PubMed:24952722).
Similarity: Both the JmjC domain and the JmjN domain are required for enzymatic activity. However ARID and PHD-type 1 domain are not required for activity per se but contributed to recognition of the H3(1-21)K4me2 substrate peptide.The 2 first PHD-type zinc finger domains are required for transcription repression activity. Belongs to the JARID1 histone demethylase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 176 kDa
NCBI Official Full Name
lysine-specific demethylase 5B isoform 1
NCBI Official Synonym Full Names
lysine demethylase 5B
NCBI Official Symbol
KDM5B
NCBI Official Synonym Symbols
CT31; PLU1; PUT1; MRT65; PLU-1; JARID1B; PPP1R98; RBP2-H1; RBBP2H1A
NCBI Protein Information
lysine-specific demethylase 5B
UniProt Protein Name
Lysine-specific demethylase 5B
UniProt Gene Name
KDM5B
UniProt Synonym Gene Names
JARID1B; PLU1; RBBP2H1; CT31; RBP2-H1
NCBI Description
This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]
Uniprot Description
Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code (PubMed:24952722, PubMed:27214403, PubMed:28262558). Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5 (PubMed:24952722). In contrast, may act as a tumor suppressor for melanoma. Represses the CLOCK-ARNTL/BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 ().
Research Articles on JAD1B
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Product Notes
The JAD1B kdm5b (Catalog #AAA9604061) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JAD1B Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's JAD1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the JAD1B kdm5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JAD1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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