Rabbit anti-Human ITPKC Polyclonal Antibody | anti-ITPKC antibody

ITPKC, NT (ITPKC, IP3KC, Inositol-trisphosphate 3-kinase C, Inositol 1,4,5-trisphosphate 3-kinase C) (FITC)

Cat. Num. AAA6311436

• Gene Names: ITPKC; IP3KC; IP3-3KC
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ITPKC; Polyclonal Antibody; NT (ITPKC; IP3KC; Inositol-trisphosphate 3-kinase C; Inositol 1; 4; 5-trisphosphate 3-kinase C) (FITC); 5-trisphosphate 3-kinase C; anti-ITPKC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ITPKC antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ITPKC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96~125 amino acids from the N-terminal region of human ITPKC.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ITPKC Antibody (N-term) in HepG2 cell line lysates (35ug/lane). ITPKC (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ITPKC Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ITPKC Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ITPKC antibody

ITPKC encodes a member of the inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)] 3-kinase family of enzymes that catalyze the phosphorylation of inositol 1,4,5-trisphosphate to 1,3,4,5-tetrakisphosphate. The encoded protein is localized to the nucleus and cytoplasm and has both nuclear import and nuclear export activity. Single nucleotide polymorphisms in this gene are associated with Kawasaki disease.

Product Categories/Family for anti-ITPKC antibody

Antibodies; Protein Kinases

References

Chi, H. Hum. Mol. Genet. 19 (6), 1147-1151 (2010). Onouchi, Y. Nat. Genet. 40 (1), 35-42 (2008). Nalaskowski, M.M. Biol. Chem. 387 (5), 583-593 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 18643383
• NCBI GeneID: 80271
• NCBI Accession #: NP_079470.1
• NCBI GenBank Nucleotide #: NM_025194.2
• UniProt Accession #: Q96DU7; Q9UE25; Q9Y475
• Molecular Weight: 75,207 Da
• NCBI Official Full Name: inositol-trisphosphate 3-kinase C
• NCBI Official Synonym Full Names: inositol-trisphosphate 3-kinase C
• NCBI Official Symbol: ITPKC
• NCBI Official Synonym Symbols: IP3KC; IP3-3KC
• NCBI Protein Information: inositol-trisphosphate 3-kinase C; IP3K C; IP3 3-kinase C; InsP 3 kinase C; insP 3-kinase C; inositol 1,4,5-trisphosphate 3-kinase C
• UniProt Protein Name: Inositol-trisphosphate 3-kinase C
• Protein Family: Inositol-trisphosphate 3-kinase
• UniProt Gene Name: ITPKC
• UniProt Synonym Gene Names: IP3KC; IP3 3-kinase C; IP3K C
• UniProt Entry Name: IP3KC_HUMAN

NCBI Description

This gene encodes a member of the inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)] 3-kinase family of enzymes that catalyze the phosphorylation of inositol 1,4,5-trisphosphate to 1,3,4,5-tetrakisphosphate. The encoded protein is localized to the nucleus and cytoplasm and has both nuclear import and nuclear export activity. Single nucleotide polymorphisms in this gene are associated with Kawasaki disease.[provided by RefSeq, Sep 2009]

Uniprot Description

ITPKC: Can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate. Defects in ITPKC are a cause of Kawasaki disease (KWD); also known as mucocutaneous lymph node syndrome or infantile polyarteritis. Kawasaki disease is an acute, self- limited vasculitis of infants and children characterized by prolonged fever unresponsive to antibiotics, polymorphous skin rash, erythema of the oral mucosa, lips, and tongue, erythema of the palms and soles, bilateral conjunctival injection, and cervical lymphadenopathy. Coronary artery aneurysms develop in 15 to 25% of those left untreated, making Kawasaki disease the leading cause of acquired heart disease among children in developed countries. Belongs to the inositol phosphokinase (IPK) family.

Protein type: Motility/polarity/chemotaxis; Kinase, lipid; EC 2.7.1.127

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: nucleus; cytosol

Molecular Function: calmodulin binding; inositol trisphosphate 3-kinase activity; ATP binding

Biological Process: inositol phosphate metabolic process; phosphorylation

Disease: Kawasaki Disease