Rabbit anti-Mouse ITM2B Polyclonal Antibody | anti-ITM2B antibody

ITM2B, CT (ITM2B, BRI, BRI2, Integral membrane protein 2B, Immature BRI2, Protein E25B, Transmembrane protein BRI, BRI2, membrane form, Mature BRI2, BRI2 intracellular domain, BRI2C, soluble form, Bri23 peptide, ABri23, C-terminal peptide, P23 peptide) (A

Cat. Num. AAA6311371

• Gene Names: ITM2B; BRI; FBD; ABRI; BRI2; E25B; E3-16; RDGCA; imBRI2; BRICD2B
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: ITM2B; Polyclonal Antibody; CT (ITM2B; BRI; BRI2; Integral membrane protein 2B; Immature BRI2; Protein E25B; Transmembrane protein BRI; membrane form; Mature BRI2; BRI2 intracellular domain; BRI2C; soluble form; Bri23 peptide; ABri23; C-terminal peptide; P23 peptide) (A; P23 peptide; anti-ITM2B antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ITM2B. Species crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ITM2B antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 218-247 in the C-terminal region of human ITM2B, UniProt Accession #Q9Y287.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(ITM2B Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ITM2B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(ITM2B Antibody (C-term) western blot analysis in mouse cerebellum,lung tissue lysates (35ug/lane).This demonstrates the ITM2B antibody detected the ITM2B protein (arrow).)

Related Product Information for anti-ITM2B antibody

Amyloid precursor proteins are processed by beta-secretase and gamma-secretase to produce beta-amyloid peptides which form the characteristic plaques of Alzheimer disease. This gene encodes a transmembrane protein which is processed at the C-terminus by furin or furin-like proteases to produce a small secreted peptide which inhibits the deposition of beta-amyloid. Mutations which result in extension of the C-terminal end of the encoded protein, thereby increasing the size of the secreted peptide, are associated with two neurogenerative diseases, familial British dementia and familial Danish dementia.

Product Categories/Family for anti-ITM2B antibody

Antibodies; Transmembrane Proteins

References

Peng, S., et al. Biochem. Biophys. Res. Commun. 393(3):356-361(2010). Matsuda, S., et al. J. Biol. Chem. 284(23):15815-15825(2009). Matsuda, S., et al. Mol Neurodegener 4, 41 (2009): Tsachaki, M., et al. Biotechnol J 3(12):1548-1554(2008). Kim, J., et al. J. Neurosci. 28(23):6030-6036(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 11527402
• NCBI GeneID: 9445
• NCBI Accession #: NP_068839.1
• NCBI GenBank Nucleotide #: NM_021999.4
• UniProt Accession #: Q9Y287; Q5W0A3; Q96B24; Q9NYH1
• Molecular Weight: 18,283 Da
• NCBI Official Full Name: integral membrane protein 2B
• NCBI Official Synonym Full Names: integral membrane protein 2B
• NCBI Official Symbol: ITM2B
• NCBI Official Synonym Symbols: BRI; FBD; ABRI; BRI2; E25B; E3-16; RDGCA; imBRI2; BRICD2B
• NCBI Protein Information: integral membrane protein 2B; immature BRI2; ABri/ADan amyloid peptide; transmembrane protein BRI; BRICHOS domain containing 2B
• UniProt Protein Name: Integral membrane protein 2B
• Protein Family: Integral membrane protein
• UniProt Gene Name: ITM2B
• UniProt Synonym Gene Names: BRI; BRI2; imBRI2; Bri; mBRI2; BRI2 ICD; Bri2-23
• UniProt Entry Name: ITM2B_HUMAN

NCBI Description

Amyloid precursor proteins are processed by beta-secretase and gamma-secretase to produce beta-amyloid peptides which form the characteristic plaques of Alzheimer disease. This gene encodes a transmembrane protein which is processed at the C-terminus by furin or furin-like proteases to produce a small secreted peptide which inhibits the deposition of beta-amyloid. Mutations which result in extension of the C-terminal end of the encoded protein, thereby increasing the size of the secreted peptide, are associated with two neurogenerative diseases, familial British dementia and familial Danish dementia. [provided by RefSeq, Oct 2009]

Uniprot Description

ITM2B: Plays a regulatory role in the processing of the beta- amyloid A4 precursor protein (APP) and acts as an inhibitor of the beta-amyloid peptide aggregation and fibrils deposition. Plays a role in the induction of neurite outgrowth. Functions as a protease inhibitor by blocking access of secretases to APP cleavage sites. Defects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 1 (CAA-ITM2B1). A disorder characterized by amyloid deposition in the walls of cerebral blood vessels and neurodegeneration in the central nervous system. Cerebral amyloid angiopathy, non-neuritic and perivascular plaques and neurofibrillary tangles are the predominant pathological lesions. Clinical features include progressive mental deterioration, spasticity and muscular rigidity. ABri amyloidogenic peptide variant is cleaved at the normal furin processing site to generate peptide that accumulates in the brain and produces amyloid fibrils responsible for neuronal dysfunction and dementia. ABri peptide variant forms fibrila in vitro. Defects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 2 (CAA-ITM2B2); also known as heredopathia ophthalmo-oto-encephalica. A disorder characterized by amyloid deposition in the walls of the blood vessels of the cerebrum, choroid plexus, cerebellum, spinal cord and retina. Plaques and neurofibrillary tangles are observed in the hippocampus. Clinical features include progressive ataxia, dementia, cataracts and deafness. ADan amyloidogenic peptide variant is cleaved at the normal furin processing site to generate peptide that accumulates in the brain and produces amyloid fibrils responsible for neuronal dysfunction and dementia. Belongs to the ITM2 family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: Golgi apparatus; extracellular space; intracellular membrane-bound organelle; membrane; plasma membrane; extracellular region; endosome membrane

Molecular Function: protein binding; beta-amyloid binding; ATP binding

Biological Process: nervous system development; negative regulation of amyloid precursor protein biosynthetic process

Disease: Cerebral Amyloid Angiopathy, Itm2b-related, 2; Cerebral Amyloid Angiopathy, Itm2b-related, 1; Retinal Dystrophy With Inner Retinal Dysfunction And Ganglion Cell Abnormalities