Rabbit anti-Human ITK Polyclonal Antibody | anti-ITK antibody

ITK, NT (ITK, EMT, LYK, Tyrosine-protein kinase ITK/TSK, Interleukin-2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase Lyk)

Cat. Num. AAA6011999

• Gene Names: ITK; EMT; LYK; LPFS1; PSCTK2
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ITK; Polyclonal Antibody; NT (ITK; EMT; LYK; Tyrosine-protein kinase ITK/TSK; Interleukin-2-inducible T-cell kinase; Kinase EMT; T-cell-specific kinase; Tyrosine-protein kinase Lyk); Anti -ITK; anti-ITK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ITK antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: ITK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human ITK.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of A549 cell line lysates (35ug/lane) using MBS6011999. This demonstrates MBS6011999 detected the ITK protein (arrow).)

Related Product Information for anti-ITK antibody

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq].

Product Categories/Family for anti-ITK antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 399658
• NCBI GeneID: 3702
• UniProt Accession #: Q08881; Q32ML7; B2R752
• Molecular Weight: 71,831 Da
• NCBI Official Full Name: ITK
• NCBI Official Synonym Full Names: IL2-inducible T-cell kinase
• NCBI Official Symbol: ITK
• NCBI Official Synonym Symbols: EMT; LYK; LPFS1; PSCTK2
• NCBI Protein Information: tyrosine-protein kinase ITK/TSK; kinase EMT; T-cell-specific kinase; tyrosine-protein kinase LYK; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; homolog of mouse T-cell itk/tsk; interleukin-2-inducible T cell kinase; interleukin-2-inducible T-cell kinase
• UniProt Protein Name: Tyrosine-protein kinase ITK/TSK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ITK
• UniProt Synonym Gene Names: EMT; LYK; IL-2-inducible T-cell kinase
• UniProt Entry Name: ITK_HUMAN

NCBI Description

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

ITK: an intracellular tyrosine kinase of the Tec family expressed in T-cells. The protein contains both SH2 and SH3 domains and plays a role in T-cell proliferation and differentiation. Itk-deficient murine T cells display defects in TCR/CD3-induced actin polymerization.

Protein type: Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; TK group; Tec family

Chromosomal Location of Human Ortholog: 5q31-q32

Cellular Component: extrinsic to internal side of plasma membrane; intercellular junction; cytosol

Molecular Function: protein binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: adaptive immune response; T cell activation; cytokine production; signal transduction; T cell receptor signaling pathway; regulation of cell proliferation; B cell receptor signaling pathway; interleukin-4 production; phospholipase C activation; interferon-gamma production; cellular defense response; innate immune response; NK T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Lymphoproliferative Syndrome 1