Rabbit anti-Human ITGB7 Polyclonal Antibody | anti-ITGB7 antibody

ITGB7, CT (ITGB7, Integrin beta-7, Gut homing receptor beta subunit) (Azide free) (HRP)

Cat. Num. AAA6311316

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ITGB7; Polyclonal Antibody; CT (ITGB7; Integrin beta-7; Gut homing receptor beta subunit) (Azide free) (HRP); Gut homing receptor beta subunit; anti-ITGB7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ITGB7 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ITGB7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 736-765 amino acids from the C-terminal region of human ITGB7.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ITGB7 Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the ITGB7 antibody detected the ITGB7 protein (arrow).)

Related Product Information for anti-ITGB7 antibody

Integrin alpha-4/beta-7 (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT). Integrin alpha-4/beta-7 interacts with the cell surface adhesion molecules MADCAM1 which is normally expressed by the vascular endothelium of the gastrointestinal tract. Interacts also with VCAM1 and fibronectin, an extracellular matrix component. It recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Interactions involves the tripeptide L-D-T in MADCAM1, and L-D-V in fibronectin. Binds to HIV-1 gp120, thereby allowing the virus to enter GALT, which is thought to be the major trigger of AIDS disease. Interaction would involve a tripeptide L-D-I in HIV-1 gp120. Integrin alpha-E/beta-7 (HML-1) is a receptor for E-cadherin.

Product Categories/Family for anti-ITGB7 antibody

Antibodies; Integrin

References

Cicala, C., et al. Proc. Natl. Acad. Sci. U.S.A. 106(49):20877-20882(2009). Pentikainen, U., et al. J. Mol. Biol. 393(3):644-657(2009). Lad, Y., et al. J. Biol. Chem. 283(50):35154-35163(2008). Monteiro, P., et al. Biochem. Biophys. Res. Commun. 358(2):442-448(2007). Le Floc'h, A., et al. J. Exp. Med. 204(3):559-570(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4504777
• NCBI GeneID: 3695
• NCBI Accession #: NP_000880.1
• NCBI GenBank Nucleotide #: NM_000889.2
• UniProt Accession #: P26010; Q9UCP7; Q9UCS7
• Molecular Weight: 71,582 Da
• NCBI Official Full Name: integrin beta-7
• NCBI Official Synonym Full Names: integrin subunit beta 7
• NCBI Official Symbol: ITGB7
• NCBI Protein Information: integrin beta-7
• UniProt Protein Name: Integrin beta-7
• Protein Family: Integrin
• UniProt Gene Name: ITGB7

NCBI Description

This gene encodes a protein that is a member of the integrin superfamily. Members of this family are adhesion receptors that function in signaling from the extracellular matrix to the cell. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The encoded protein forms dimers with an alpha4 chain or an alphaE chain and plays a role in leukocyte adhesion. Dimerization with alpha4 forms a homing receptor for migration of lymphocytes to the intestinal mucosa and Peyer's patches. Dimerization with alphaE permits binding to the ligand epithelial cadherin, a calcium-dependent adhesion molecule. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]

Uniprot Description

ITGB7: integrin alpha-4/beta-7 (Peyer's patches-specific homing receptor lpam-1) is expected to play a role in adhesive interactions of leukocytes. It is a receptor for fibronectin and recognizes one or more domains within the alternatively spliced CS-1 region of fibronectin. Integrin alpha-4/beta-7 is also a receptor for MADCAM1 and VCAM1. It recognizes the sequence L-D-T in MADCAM 1. Integrin alpha-e/beta-7 (HML-1) is a receptor for e-cadherin. Two alternatively spliced isoforms have been described.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cell surface; integrin complex; membrane; plasma membrane; receptor complex

Molecular Function: cell adhesion molecule binding; protein binding

Biological Process: cell adhesion; cell-matrix adhesion; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; integrin-mediated signaling pathway; leukocyte tethering or rolling; receptor clustering; regulation of immune response