Rabbit anti-Human ITGB1BP1 Polyclonal Antibody | anti-ITGB1BP1 antibody

ITGB1BP1 Antibody

Cat. Num. AAA7113078

• Gene Names: ITGB1BP1; ICAP1; ICAP1A; ICAP1B; ICAP-1A; ICAP-1B; ICAP-1alpha
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%; Protein G Purified
• Synonyms: ITGB1BP1; Polyclonal Antibody; ITGB1BP1 Antibody; Integrin beta-1-binding protein 1; Integrin cytoplasmic domain-associated protein 1; ICAP-1; ICAP1; anti-ITGB1BP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%; Protein G Purified
• Form/Format: Liquid
• Sequence Length: 200

• Applicable Applications for anti-ITGB1BP1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000-1:5000; IHC: 1:200-1:500
• Immunogen Species: Human
• Immunogen: Recombinant Human Integrin beta-1-binding protein 1 protein (1-200AA)
• Conjugate: Non-Conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysateAll lanes: ITGB1BP1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 22, 17 kDaObserved band size: 22 kDa)

Immunohistochemistry (IHC)

(IHC image of CSB-PA011881LA01HU diluted at 1:300 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Product Categories/Family for anti-ITGB1BP1 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 975830185
• NCBI GeneID: 9270
• NCBI Accession #: NP_001305995.1
• NCBI GenBank Nucleotide #: NM_001319066.1
• UniProt Accession #: O14713; O14714; Q53RS0; D6W4Y9
• Molecular Weight: 16,140 Da
• NCBI Official Full Name: integrin beta-1-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: integrin subunit beta 1 binding protein 1
• NCBI Official Symbol: ITGB1BP1
• NCBI Official Synonym Symbols: ICAP1; ICAP1A; ICAP1B; ICAP-1A; ICAP-1B; ICAP-1alpha
• NCBI Protein Information: integrin beta-1-binding protein 1
• UniProt Protein Name: Integrin beta-1-binding protein 1
• Protein Family: Integrin beta-1-binding protein
• UniProt Gene Name: ITGB1BP1
• UniProt Synonym Gene Names: ICAP1; ICAP-1

NCBI Description

The cytoplasmic domains of integrins are essential for cell adhesion. The protein encoded by this gene binds to the beta1 integrin cytoplasmic domain. The interaction between this protein and beta1 integrin is highly specific. Two isoforms of this protein are derived from alternatively spliced transcripts. The shorter form of this protein does not interact with the beta1 integrin cytoplasmic domain. The longer form is a phosphoprotein and the extent of its phosphorylation is regulated by the cell-matrix interaction, suggesting an important role of this protein during integrin-dependent cell adhesion. Several transcript variants, some protein-coding and some non-protein coding, have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

Key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail and preventing the activation of integrin alpha-5/beta-1 (heterodimer of ITGA5 and ITGB1) by talin or FERMT1. Plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. Stimulates cellular proliferation in a fibronectin-dependent manner. Involved in the regulation of beta-1 integrin-containing focal adhesion (FA) site dynamics by controlling its assembly rate during cell adhesion; inhibits beta-1 integrin clustering within FA by directly competing with talin TLN1, and hence stimulates osteoblast spreading and migration in a fibronectin-and/or collagen-dependent manner. Acts as a guanine nucleotide dissociation inhibitor (GDI) by regulating Rho family GTPases during integrin-mediated cell matrix adhesion; reduces the level of active GTP-bound form of both CDC42 and RAC1 GTPases upon cell adhesion to fibronectin. Stimulates the release of active CDC42 from the membranes to maintain it in an inactive cytoplasmic pool. Participates in the translocation of the Rho-associated protein kinase ROCK1 to membrane ruffles at cell leading edges of the cell membrane, leading to an increase of myoblast cell migration on laminin. Plays a role in bone mineralization at a late stage of osteoblast differentiation; modulates the dynamic formation of focal adhesions into fibrillar adhesions, which are adhesive structures responsible for fibronectin deposition and fibrillogenesis. Plays a role in blood vessel development; acts as a negative regulator of angiogenesis by attenuating endothelial cell proliferation and migration, lumen formation and sprouting angiogenesis by promoting AKT phosphorylation and inhibiting ERK1/2 phosphorylation through activation of the Notch signaling pathway. Promotes transcriptional activity of the MYC promoter.