Rabbit ITGA7 Polyclonal Antibody | anti-ITGA7 antibody

ITGA7 Antibody

Cat. Num. AAA9629239

• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ITGA7; Polyclonal Antibody; ITGA7 Antibody; alpha 7; FLJ25220; Integrin alpha 7; Integrin alpha 7 chain; Integrin alpha 7 heavy chain; Integrin alpha 7 light chain; Integrin alpha-7 70 kDa form; ITA7_HUMAN; ITGA 7; Itga7; anti-ITGA7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ITGA7 Antibody detects endogenous levels of total ITGA7.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ITGA7 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: Belongs to the integrin alpha chain family.
• Post Translational Modifications: ADP-ribosylated on at least two sites of the extracellular domain in skeletal myotubes.A 70 kDa form is created by proteolytic cleavage. Cleavage is elevated during myogenic differentiation and the cleaved form enhances cell adhesion and spreading on laminin.
• Subunit Structure: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-7 associates with beta-1. Interacts with COMP (By similarity). Interacts (via C-terminus intracellular tail region) with CIB1; the interaction is stabilized/increased in a calcium- and magnesium-dependent manner.
• Tissue Specificity: Isoforms containing segment A are predominantly expressed in skeletal muscle. Isoforms containing segment B are abundantly expressed in skeletal muscle, moderately in cardiac muscle, small intestine, colon, ovary and prostate and weakly in lung and testes. Isoforms containing segment X2D are expressed at low levels in fetal and adult skeletal muscle and in cardiac muscle, but are not detected in myoblasts and myotubes. In muscle fibers isoforms containing segment A and B are expressed at myotendinous and neuromuscular junctions; isoforms containing segment C are expressed at neuromuscular junctions and at extrasynaptic sites. Isoforms containing segments X1 or X2 or, at low levels, X1X2 are expressed in fetal and adult skeletal muscle (myoblasts and myotubes) and cardiac muscle.
• Function: Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation (By similarity). Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-ITGA7 antibody

Membrane; Single-pass type I membrane protein; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 222418613
• NCBI GeneID: 3679
• NCBI Accession #: NP_001138468.1
• NCBI GenBank Nucleotide #: NM_001144996.1
• UniProt Accession #: Q13683; O43197; Q86W93; Q9NY89; Q9UET0; Q9UEV2; B4E3U0; C9JMD3; C9JMZ6
• Molecular Weight: 128,948 Da
• NCBI Official Full Name: integrin alpha-7 isoform 1
• NCBI Official Synonym Full Names: integrin, alpha 7
• NCBI Official Symbol: ITGA7
• NCBI Protein Information: integrin alpha-7; integrin alpha 7 chain
• UniProt Protein Name: Integrin alpha-7
• Protein Family: Integrin
• UniProt Gene Name: ITGA7
• UniProt Entry Name: ITA7_HUMAN

NCBI Description

The protein encoded by this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. They mediate a wide spectrum of cell-cell and cell-matrix interactions, and thus play a role in cell migration, morphologic development, differentiation, and metastasis. This protein functions as a receptor for the basement membrane protein laminin-1. It is mainly expressed in skeletal and cardiac muscles and may be involved in differentiation and migration processes during myogenesis. Defects in this gene are associated with congenital myopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

ITGA7: Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation. Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells. Defects in ITGA7 are the cause of muscular dystrophy congenital due to integrin alpha-7 deficiency (MDCI). A form of congenital muscular dystrophy. Patients present at birth, or within the first few months of life, with hypotonia, muscle weakness and often with joint contractures. Belongs to the integrin alpha chain family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: muscle tendon junction; cell surface; cytoplasm; plasma membrane; neuromuscular junction; sarcolemma

Molecular Function: integrin binding; protein heterodimerization activity; metal ion binding; laminin binding

Biological Process: integrin-mediated signaling pathway; regulation of cell shape; heterotypic cell-cell adhesion; muscle development; cell migration; skeletal muscle development; extracellular matrix organization and biogenesis; cell-matrix adhesion; blood vessel morphogenesis

Disease: Muscular Dystrophy, Congenital, Due To Integrin Alpha-7 Deficiency