Rabbit ITGA3 / CD49c Polyclonal Antibody | anti-ITGA3 antibody
Rabbit Polyclonal to Human ITGA3 / CD49c
Predicted Reactivity: Monkey, Mouse, Bat, Dog, Hamster, Horse
Predicted Reactivity: Monkey, Mouse, Bat, Dog, Hamster, Horse
Tissue Preparation:
Formalin fixation and embedding in paraffin wax.
Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.
Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT
Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT
Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT
Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT
Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the family of integrins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain, and function as cell surface adhesion molecules. This gene encodes alpha 3 subunit, which undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 subunit to form an integrin that interacts with many extracellular-matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]
Uniprot Description
ITGA3: an integral membrane protein that heterodimerizes with a beta chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. Undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1. Two alternatively spliced isoforms have been described.
Protein type: Cell adhesion; Receptor, misc.; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q21.33
Cellular Component: filopodium membrane; focal adhesion; cell surface; growth cone; perinuclear region of cytoplasm; basolateral plasma membrane; plasma membrane; excitatory synapse; integrin complex; receptor complex; external side of plasma membrane
Molecular Function: integrin binding; collagen binding; protein domain specific binding; protein binding; protease binding; protein heterodimerization activity; metal ion binding; fibronectin binding; laminin binding; glycoprotein binding
Biological Process: skin development; response to drug; integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; maternal process involved in pregnancy; heart development; cell-matrix adhesion; regulation of BMP signaling pathway; regulation of Wnt receptor signaling pathway; neuron migration; mesodermal cell differentiation; memory; regulation of transforming growth factor beta receptor signaling pathway; negative regulation of Rho protein signal transduction; blood coagulation; leukocyte migration; negative regulation of cell projection organization and biogenesis; lung development
Disease: Interstitial Lung Disease, Nephrotic Syndrome, And Epidermolysis Bullosa, Congenital
Research Articles on ITGA3
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Product Notes
The ITGA3 itga3 (Catalog #AAA243401) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human ITGA3 / CD49c reacts with Bovine, Gorilla, Human Predicted Reactivity: Monkey, Mouse, Bat, Dog, Hamster, Horse and may cross-react with other species as described in the data sheet. AAA Biotech's ITGA3 / CD49c can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin). IHC-P (7.5 - 10 ug/ml). Researchers should empirically determine the suitability of the ITGA3 itga3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITGA3 / CD49c, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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