Western Blot (WB)
(Western Blot analysis of ISM2 expression in transfected 293T cell line by ISM2 polyclonal antibody. Lane 1: THSD3 transfected lysate (53.13kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human ISM2 Polyclonal Antibody | anti-ISM2 antibody
ISM2 (Isthmin-2, Thrombospondin and AMOP Domain-containing Isthmin-like Protein 1, Thrombospondin Type-1 Domain-containing Protein 3, TAIL1, THSD3, PSEC0137, DKFZp686E0215, FLJ32147, MGC119416)
Gene Names
ISM2; TAIL1; THSD3
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
ISM2; Polyclonal Antibody; ISM2 (Isthmin-2; Thrombospondin and AMOP Domain-containing Isthmin-like Protein 1; Thrombospondin Type-1 Domain-containing Protein 3; TAIL1; THSD3; PSEC0137; DKFZp686E0215; FLJ32147; MGC119416); Anti -ISM2 (Isthmin-2; anti-ISM2 antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ISM2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MTPGNATPPRTPEVTPLRLELQKLPGLANTTLSTPNPDTQASASPDPRPLREEEEARLLPRTHLQAELHQHGCWTVTEPAALTPGNATPPRTQEVTPLLLELQKLPELVHATLSTPNPDNQVTIKVVEDPQAEVSIDLLAEPSNPPPQDTLSWLPALWSFLWGDYKGEEKDRAPGEKGEEKEEDEDYPSEDIEGEDQEDKEEDEEEQALWFNGTTDNWDQGWLAPGDWVFKDSVSYDYEPQKEWSPWSPCSGNCSTGKQQRTRPCGYGCTATETRTCDLPSCPGTEDKDTLGLPSEEWKLLARNATDMHDQDVDSCEKWLNCKSDFLIKYLSQMLRDLPSCPCAYPLEAMDSPVSLQDEHQGRSFRWRDASGPRERLDIYQPTARFCLRSMLSGESSTLAAQHCCYDEDSRLLTRGKGAGMPNLISTDFSPKLHFKFDTTPWILCKGDWSRLHAVLPPNNGRACTDNPLEEEYLAQLQEAKEY
Applicable Applications for anti-ISM2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 10ug/ml
Dilution: Immunofluorescence: 10ug/ml
Immunogen
Full length human ISM2, aa1-483 (NP_954874.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of ISM2 expression in transfected 293T cell line by ISM2 polyclonal antibody. Lane 1: THSD3 transfected lysate (53.13kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of ISM2 expression in transfected 293T cell line by ISM2 polyclonal antibody. Lane 1: THSD3 transfected lysate (53.13kD). Lane 2: Non-transfected lysate.)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to THSD3 on HeLa cell. [antibody concentration 10ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to THSD3 on HeLa cell. [antibody concentration 10ug/ml].)
Product Categories/Family for anti-ISM2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
63,906 Da
NCBI Official Full Name
ISM2 protein
NCBI Official Synonym Full Names
isthmin 2
NCBI Official Symbol
ISM2
NCBI Official Synonym Symbols
TAIL1; THSD3
NCBI Protein Information
isthmin-2; isthmin 2 homolog; thrombospondin, type I domain-containing 3; thrombospondin, type I, domain containing 3; thrombospondin and AMOP containing isthmin-like 1; thrombospondin type-1 domain-containing protein 3; thrombospondin and AMOP domain-containing isthmin-like protein 1
UniProt Protein Name
Isthmin-2
Protein Family
UniProt Gene Name
ISM2
UniProt Synonym Gene Names
TAIL1; THSD3
UniProt Entry Name
ISM2_HUMAN
NCBI Description
The protein encoded by this gene contains a type 1 thrombospondin domain, which is present in thrombospondin, a number of proteins involved in the complement pathway, as well as in extracellular matrix proteins. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Mar 2009]
Uniprot Description
THSD3: contains a type 1 thrombospondin domain, which is present in thrombospondin, a number of proteins involved in the complement pathway, as well as in extracellular matrix proteins. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Mar 2009]
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: extracellular region
Research Articles on ISM2
Similar Products
Product Notes
The ISM2 ism2 (Catalog #AAA6009899) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ISM2 (Isthmin-2, Thrombospondin and AMOP Domain-containing Isthmin-like Protein 1, Thrombospondin Type-1 Domain-containing Protein 3, TAIL1, THSD3, PSEC0137, DKFZp686E0215, FLJ32147, MGC119416) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ISM2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence and Western Blot. Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the ISM2 ism2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MTPGNATPPR TPEVTPLRLE LQKLPGLANT TLSTPNPDTQ ASASPDPRPL REEEEARLLP RTHLQAELHQ HGCWTVTEPA ALTPGNATPP RTQEVTPLLL ELQKLPELVH ATLSTPNPDN QVTIKVVEDP QAEVSIDLLA EPSNPPPQDT LSWLPALWSF LWGDYKGEEK DRAPGEKGEE KEEDEDYPSE DIEGEDQEDK EEDEEEQALW FNGTTDNWDQ GWLAPGDWVF KDSVSYDYEP QKEWSPWSPC SGNCSTGKQQ RTRPCGYGCT ATETRTCDLP SCPGTEDKDT LGLPSEEWKL LARNATDMHD QDVDSCEKWL NCKSDFLIKY LSQMLRDLPS CPCAYPLEAM DSPVSLQDEH QGRSFRWRDA SGPRERLDIY QPTARFCLRS MLSGESSTLA AQHCCYDEDS RLLTRGKGAG MPNLISTDFS PKLHFKFDTT PWILCKGDWS RLHAVLPPNN GRACTDNPLE EEYLAQLQEA KEY. It is sometimes possible for the material contained within the vial of "ISM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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