Immunofluorescence (IF)
(Fluorescent image of HUVEC cell stained with ISL2 Antibody (N-term) .HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ISL2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).ISL2 immunoreactivity is localized to Nucleus significantly.)
Rabbit anti-Human ISL2 Polyclonal Antibody | anti-ISL2 antibody
ISL2, NT (ISL2, Insulin gene enhancer protein ISL-2)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunofluorescence (IF)
(Fluorescent image of HUVEC cell stained with ISL2 Antibody (N-term) .HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ISL2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).ISL2 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent image of HUVEC cell stained with ISL2 Antibody (N-term) .HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ISL2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).ISL2 immunoreactivity is localized to Nucleus significantly.)
Western Blot (WB)
(ISL2 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ISL2 antibody detected the ISL2 protein (arrow).)
Western Blot (WB)
(ISL2 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ISL2 antibody detected the ISL2 protein (arrow).)
NCBI and Uniprot Product Information
Uniprot Description
islet-2: Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 15q23
Cellular Component: nucleus
Molecular Function: DNA binding; zinc ion binding; sequence-specific DNA binding
Biological Process: peripheral nervous system neuron development; negative regulation of neuron differentiation; visceral motor neuron differentiation; regulation of transcription, DNA-dependent; spinal cord motor neuron cell fate specification; neuron development; retinal ganglion cell axon guidance
Research Articles on ISL2
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Product Notes
The ISL2 isl2 (Catalog #AAA644739) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ISL2, NT (ISL2, Insulin gene enhancer protein ISL-2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ISL2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the ISL2 isl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ISL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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