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Western Blot (WB) (Western blot analysis of lysate from rat brain tissue lysate, using Isl1 Antibody (C-term). MBS9208120 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

Rabbit Isl1 Polyclonal Antibody | anti-Isl1 antibody

Mouse Isl1 Antibody (C-term)

Reactivity
Rat (Predicted Reactivity: Chicken, Zebrafish, Human)
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Isl1; Polyclonal Antibody; Mouse Isl1 Antibody (C-term); Insulin gene enhancer protein ISL-1; Islet-1; anti-Isl1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat (Predicted Reactivity: Chicken, Zebrafish, Human)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Isl1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 302-336 amino acids from the C-terminal region of mouse Isl1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
326
Applicable Applications for anti-Isl1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysate from rat brain tissue lysate, using Isl1 Antibody (C-term). MBS9208120 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

Western Blot (WB) (Western blot analysis of lysate from rat brain tissue lysate, using Isl1 Antibody (C-term). MBS9208120 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Related Product Information for anti-Isl1 antibody
Binds and regulates the promoters of the insulin, glucagon and somatostatin genes. Involved in the specificarion of motor neurons in cooperation with LHX3 and LDB1.
Product Categories/Family for anti-Isl1 antibody
References
Ando K.,et al.J. Mol. Endocrinol. 31:419-425(2003).
Koehler K.,et al.Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
Bhati M.,et al.EMBO J. 27:2018-2029(2008).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39036
NCBI Official Full Name
insulin gene enhancer protein ISL-1
NCBI Official Synonym Full Names
ISL1 transcription factor, LIM/homeodomain
NCBI Official Symbol
Isl1
NCBI Protein Information
insulin gene enhancer protein ISL-1
UniProt Protein Name
Insulin gene enhancer protein ISL-1
UniProt Gene Name
Isl1
UniProt Synonym Gene Names
Islet-1
UniProt Entry Name
ISL1_MOUSE

Uniprot Description

ISL1: Binds to one of the cis-acting domain of the insulin gene enhancer.

Protein type: Nuclear receptor co-regulator; DNA-binding; Cell development/differentiation; Motility/polarity/chemotaxis; Transcription factor

Cellular Component: nucleoplasm; mitochondrion; intracellular; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; metal ion binding; bHLH transcription factor binding; estrogen receptor binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; heart morphogenesis; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of interleukin-12 production; multicellular organismal development; heart development; positive regulation of interleukin-1 alpha production; negative regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of estrogen receptor signaling pathway; neuron differentiation; regulation of transcription, DNA-dependent; pancreas development; positive regulation of cell proliferation; neuron fate specification; ventricular cardiac muscle morphogenesis; negative regulation of neuron apoptosis; sensory system development; neural crest cell migration; positive regulation of DNA binding; trigeminal nerve development; pharyngeal system development; neuron fate commitment; spinal cord motor neuron cell fate specification; positive regulation of insulin secretion; positive regulation of interleukin-6 production; spinal cord motor neuron differentiation; positive regulation of tumor necrosis factor production; peripheral nervous system neuron development; positive regulation of interleukin-1 beta production; positive regulation of angiogenesis; negative regulation of neuron differentiation; positive regulation of interferon-gamma production; mesenchymal cell differentiation; visceral motor neuron differentiation; regulation of gene expression; pituitary gland development; negative regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; peripheral nervous system neuron axonogenesis; retinal ganglion cell axon guidance

Research Articles on Isl1

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Product Notes

The Isl1 isl1 (Catalog #AAA9208120) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse Isl1 Antibody (C-term) reacts with Rat (Predicted Reactivity: Chicken, Zebrafish, Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Isl1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Isl1 isl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Isl1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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